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ARS Home » Midwest Area » Ames, Iowa » National Animal Disease Center » Virus and Prion Research » Research » Publications at this Location » Publication #369048

Research Project: Intervention Strategies to Control Endemic and New and Emerging Viral Diseases of Swine

Location: Virus and Prion Research

Title: Characterization of homologous protective humoral immunity against Seneca Valley virus

item KIM, HANJUN - Iowa State University
item Buckley, Alexandra
item GUO, BAOQING - Iowa State University
item Lager, Kelly
item YOON, KYOUNG-JIN - Iowa State University

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 7/21/2019
Publication Date: 11/4/2019
Citation: Kim, H., Buckley, A.C., Guo, B., Lager, K.M., Yoon, K. 2019. Characterization of homologous protective humoral immunity against Seneca Valley virus. Conference for Research Workers in Animal Diseases (CRWAD)2019 [abstract] p. 198.

Interpretive Summary:

Technical Abstract: Seneca Valley virus (SVV), a member of Picornaviridae, causes vesicular disease in pigs. To understand what comprises protective immunity against SVV, clinical and antibody responses over time after experimental infection was studied. Fifteen 9-month-old gilts were inoculated intranasally with a contemporary US strain of SVV. Sera were periodically collected until 33 days post inoculation (dpi). On either 111 or 124 dpi, 12 pigs were re-challenged with the same SVV strain. Besides monitoring apparent clinical signs and gross lesions, each pig was bled on 0 and 7 days post challenge (dpc) as well as on necropsy dates between 13 to 43 dpc. Antibody responses, including isotypes, were assessed by virus neutralization (VN) and indirect fluorescent antibody (IFA) tests over time before and after re-challenge. The reactivity of the antibody response with four structural proteins of SVV was characterized by Western immunoblot.