Location: Virus and Prion ResearchTitle: Characterization of homologous protective humoral immunity against Seneca Valley virus
|KIM, HANJUN - Iowa State University|
|GUO, BAOQING - Iowa State University|
|YOON, KYOUNG-JIN - Iowa State University|
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 7/21/2019
Publication Date: 11/4/2019
Citation: Kim, H., Buckley, A.C., Guo, B., Lager, K.M., Yoon, K. 2019. Characterization of homologous protective humoral immunity against Seneca Valley virus. Conference for Research Workers in Animal Diseases (CRWAD)2019 [abstract] p. 198.
Technical Abstract: Seneca Valley virus (SVV), a member of Picornaviridae, causes vesicular disease in pigs. To understand what comprises protective immunity against SVV, clinical and antibody responses over time after experimental infection was studied. Fifteen 9-month-old gilts were inoculated intranasally with a contemporary US strain of SVV. Sera were periodically collected until 33 days post inoculation (dpi). On either 111 or 124 dpi, 12 pigs were re-challenged with the same SVV strain. Besides monitoring apparent clinical signs and gross lesions, each pig was bled on 0 and 7 days post challenge (dpc) as well as on necropsy dates between 13 to 43 dpc. Antibody responses, including isotypes, were assessed by virus neutralization (VN) and indirect fluorescent antibody (IFA) tests over time before and after re-challenge. The reactivity of the antibody response with four structural proteins of SVV was characterized by Western immunoblot.