Submitted to: PLOS ONE
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/28/2020
Publication Date: 5/14/2020
Citation: Cai, G., Scofield, S.R. 2020. Mitochondrial Genome Sequence of Phytophthora sansomeana and Comparative Analysis of Phytophthora Mitochondrial Genomes. PLoS ONE. 15(5). https://doi.org/10.1371/journal.pone.0231296.
Interpretive Summary: Phytophthora sansomeana infects soybean and causes root rot. Above ground, it causes yellowing and stunting or whole-plant wilting. Molecular data about this pathogen is limited. In this study, we sequenced and annotated its mitochondrial genome and compared it to the mitochondrial genomes of nine other Phytophthora species. These mitochondrial genomes were found to be very conserved in the genes they encoded with few exceptions. Three inversions in gene orders were observed. We also used the mitochondrial data to study the evolution history of Phytophthora species. These molecular data serve as the foundation to more applied studies to control diseases caused by Phytophthora species.
Technical Abstract: hytophthora sansomeana infects soybean and causes root rot. It was recently separated from the species complex P. megasperma sensu lato. In this study, we sequenced and annotated its complete mitochondrial genome and compared it to that of nine other Phytophthora species. The genome was assembled into a circular molecule of 39,618 bp with a 22.03% G+C content. Forty-two protein coding genes, 25 tRNA genes and two rRNA genes were annotated in this genome. The protein coding genes include 14 genes in the respiratory complexes, four ATP synthetase genes, 16 ribosomal proteins genes, a tatC translocase gene, six conserved ORFs and a unique orf402. The tRNA genes encode tRNAs for 19 amino acids. Comparison among mitochondrial genomes of 10 Phytophthora species showed that these genomes were conserved in gene content with few exceptions. A 3' truncated atp9 gene was found in P. nicotianae. All 10 Phytophthora species, as well as other oomycetes and stramenopiles, lacked tRNA genes for threonine in their mitochondria. Three inversions, each covering multiple genes, were found in the mitochondria of the 10 Phytophthora species. Phylogenomic analysis using the mitochondrial genomes supported or enhanced previous findings of the phylogeny of Phytophthora spp.