Genome sequence resource for Spiroplasma citri, strain CC-2, associated with citrus stubborn disease in California

Authors: Yokomi, Raymond - Ray; Chen, Jianchi; RATTNER, RACHEL - Cooperative Agricultural Support Services; SELVARAJ, VIJAYANANDRAJ - Foreign Agricultural Service (FAS, USDA); MAJESHWARI, YOGITA - Foreign Agricultural Service (FAS, USDA); OSMAN, F - University Of California, Davis; PAGLIACICA, D - University Of California; VIDALAKIS, GEORGES - University Of California

Submitted to: Phytopathology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 9/3/2019
Publication Date: 9/10/2019
Citation: Yokomi, R.K., Chen, J., Rattner, R., Selvaraj, V., Majeshwari, Y., Osman, F., Pagliacica, D., Vidalakis, G. 2019. Genome sequence resource for Spiroplasma citri, strain CC-2, associated with citrus stubborn disease in California. Phytopathology. 110(2):254-256. https://doi.org/10.1094/PHYTO-08-19-0304-A.
DOI: https://doi.org/10.1094/PHYTO-08-19-0304-A

Interpretive Summary: Spiroplasma citri is a bacterium with a wide host range. S. citri is transmitted to citrus by the beet leafhopper and causes citrus stubborn disease that induces various symptoms such as shortened internodes, chlorotic leaves, small upturned leaves, stunted growth, off-season flowering, small misshaped fruit with stylar-end greening. S. citri was isolated from Chinese cabbage grown on a farm in Fresno, California and designated as S. citri CC-2 strain. DNA from S. citri CC-2 was extracted from a pure culture grown in liquid media, purified and subjected to PacBio sequencing. Bioinformatic analysis of the data resulted in assembly of four contigs. Contig 1 was a single circular chromosome of 1,709,192 bp and Contigs 2, 3, and 4 were plasmids of 40,210, 39,313, and 2,921 bp in size. The genome developed herein extends the sequence database of S. citri and is the first whole genome sequence record of the pathogen from California. The data are useful to understand the nature of the bacterium and development of improved methods for detection and control.

Technical Abstract: Spiroplasma citri is a bacterium that causes stubborn disease of citrus and infects other crops, ornamentals and weeds. It is transmitted by leafhoppers in a circulative and persistent manner. Due to limited sequence data on S. citri, the bacterium was isolated from naturally-infected Chinese cabbage grown on a farm in Fresno County, California. DNA from S. citri CC-2 was extracted from a pure culture in LD8 and subjected to PacBio sequencing. Four contigs were obtained with a single circular chromosome of 1,709,192 bp and three plasmids of 40,210, 39,313, and 2,921 bp in size. The genome developed herein extends the sequence database of S. citri and is the first whole genome sequence record of S. citri from California.