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ARS Home » Plains Area » Clay Center, Nebraska » U.S. Meat Animal Research Center » Reproduction Research » Research » Publications at this Location » Publication #361321

Research Project: Applying Developmental Programming to Improve Production Efficiency in Beef Cattle

Location: Reproduction Research

Title: The influence of preovulatory estradiol on trophectoderm and uterine gene transcript abundance around maternal recognition of pregnancy in beef cattle

Author
item NORTHROP, EMMA - South Dakota State University
item RICH, JERICA - South Dakota State University
item Cushman, Robert - Bob
item Miles, Jeremy
item Lindholm-Perry, Amanda
item Keel, Brittney
item YAO, R - South Dakota State University
item GE, X - South Dakota State University
item PERRY, GEORGE - South Dakota State University

Submitted to: Society for the Study of Reproduction Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 4/1/2019
Publication Date: 6/1/2019
Citation: Northrop, E.J., Rich, J.J., Cushman, R.A., Miles, J.R., Lindholm-Perry, A.K., Keel, B.N., Yao, R., Ge, X., Perry, G.A. 2019. The influence of preovulatory estradiol on trophectoderm and uterine gene transcript abundance around maternal recognition of pregnancy in beef cattle [abstract]. Society for the Study of Reproduction Annual Meeting. Abstract Program, p. 317. Available: https://community.ssr.org/news-events/past-meetings/annual-meeting575

Interpretive Summary:

Technical Abstract: In cattle, most embryonic losses occur during the first month of pregnancy. Embryo survival and pregnancy success is increased among animals that exhibit estrus prior to fixed time AI, but there are no differences in conceptus survival to d16. The objective of this study was to examine differences in uterine and conceptus transcriptomes on d16 of pregnancy based on preovulatory estradiol (E2) exposure. We hypothesized that differences in uterine environment have a greater impact on pregnancy success than differences in conceptus development on d16. Beef cows/heifers were synchronized, artificially inseminated (d0), and grouped into high (highE2: n=11) or low (lowE2: n=3) preovulatory E2. Uteri were flushed to collect d16 conceptuses, and endometrial samples were collected from the ipsilateral uterine horn. Total cellular RNA was extracted from endometrium for RNA sequencing. Real-Time PCR (RTPCR) was performed on trophectoderm (TE) RNA for relative abundance of IFNT, PTGS2, TM4SF1, C3, PPARG, FGFR2, and GAPDH. Transcript abundances in the endometrium were quantified using kallisto, differentially expressed genes (DEGs) were determined using DESeq2 (FDR <0.05, FC>2), and IPA was used for pathway analysis. RTPCR data were analyzed using the MIXED procedure in SAS. There were no differences in mRNA abundances in TE, but there were 432 DEGs among the highE2/conceptus versus lowE2/conceptus groups, 253 were downregulated (PRKCG, PRND, MRAP2) and 179 were upregulated (PRSS2, CRYGS, C1QL2) in the highE2/conceptus group. Previously, we reported 48 differentially expressed proteins in uterine luminal fluid between highE2 and lowE2 animals on d16, 4 of these were differentially expressed (FDR <0.10) at the mRNA level. Similar pathways for mRNA and proteins included: calcium signaling, protein kinase A signaling, and connective tissue development. These results demonstrate greater differences in uterine function than in conceptus developmental competence between highE2 and lowE2 animals on d16.