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Research Project: Innovative Strategies and Methods for Improving the Management, Availability, and Utility of Plant Genetic Resource Collections

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Title: Cryopreservation of grapevine shoot tips from in vitro plants using droplet vitrification and V cryo-plate techniques

Author
item PATHIRANA, R - Plant And Food Research
item Bonnart, Remi
item Shepherd, Ashley
item Volk, Gayle

Submitted to: Book Chapter
Publication Type: Book / Chapter
Publication Acceptance Date: 7/26/2019
Publication Date: 11/24/2019
Citation: Bettoni JC, Pathirana R, Bonnart R, Shepherd A, Volk G. 2019. Cryopreservation of grapevine shoot tips from in vitro plants using droplet vitrification and V cryo-plate techniques. In: Faisal M, Alatar AA, editors. Synthetic Seeds. Switzerland AG: Springer Nature p. 469-482.
DOI: https://doi.org/10.1007/978-3-030-24631-0_22

Interpretive Summary: The availability of and easy access to Vitis genetic resources are essential for future breeding program advances. Cryopreservation is currently considered an ideal means for the long-term preservation of clonally propagated plant genetic resources. When robust Vitis cryopreservation methods are available, there is an opportunity to preserve collections for extended lengths of time with minimal cost and labor requirements, and a low risk of loss. This chapter describes the droplet vitrification and V cryo-plate protocols that have been shown to be effective for the cryopreservation of multiple V. vinifera genotypes and other Vitis species.

Technical Abstract: Not required for a book chapter.