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ARS Home » Northeast Area » Washington, D.C. » National Arboretum » Floral and Nursery Plants Research » Research » Publications at this Location » Publication #359088
Research Project: Detection, Identification, and Characterization of New and Emerging Viral and Bacterial Diseases of Ornamental Plants

Location: Floral and Nursery Plants Research

Title: Five newly collected turnip mosaic virus (TuMV) isolates from Jeju Island, Korea are closely related to previously reported Korean TuMV isolates but show distinctive symptom development

Author
item HU, WEN-XING - Chungnam National University
item KIM, BYOUNG-JO - Chungnam National University
item KWAK, YOUNGHWAN - Chungnam National University
item SEO, EUN-YOUNG - Chungnam National University
item KIM, JUNG-KYU - Chungnam National University
item HAN, JAE-YEONG - Chungnam National University
item KIM, IK-HYUN - Chungnam National University
item LIM, YONGPYO - Chungnam National University
item CHO, IN-SOOK - Rural Development Administration - Korea
item Domier, Leslie
item Hammond, John
item LIM, HYOUN-SUB - Chungnam National University

Submitted to: Plant Pathology Journal
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/21/2019
Publication Date: 8/1/2019
Citation: Hu, W., Kim, B., Kwak, Y., Seo, E., Kim, J., Han, J., Kim, I., Lim, Y., Cho, I., Domier, L.L., Hammond, J., Lim, H. 2019. Five newly collected turnip mosaic virus (TuMV) isolates from Jeju Island, Korea are closely related to previously reported Korean TuMV isolates but show distinctive symptom development. Plant Pathology Journal. 35(4):381-387. https://doi.org/10.5423/PPJ.NT.11.2018.0238.
DOI: https://doi.org/10.5423/PPJ.NT.11.2018.0238

Interpretive Summary: Plant virus infections cause reductions in yield and quality in many crops, and may be spread within the crop by horticultural operations, or by insects or other vectors. New virus isolates may arise by mutation, or by movement into the crop from external sources, often resulting from long-distance movement by windblown insect vectors, or via seed transmission. Scientists in Korea discovered five new isolates of Turnip mosaic virus from Jeju Island, Korea, which were shown to be most closely related to isolates from the Korean mainland, but differed in pathogenicity. Analysis of protein sequences revealed that the isolates differed by a minimum of three amino acids between ‘mild’ and ‘severe’ symptom isolates.

Technical Abstract: For several years, temperatures in the Korean peninsula have gradually increased due to climate change, resulting in a changing environment for growth of crops and vegetables. An associated consequence is that emerging species of insect vector have caused increased viral transmission. In Jeju Island, Korea, occurrences of viral disease have increased. Here, we report characterization of five newly collected turnip mosaic virus (TuMV) isolates from a survey on Jeju Island in 2017. Their full-length sequences share 98.9-99.9% nucleotide sequence identity and were most closely related to TuMV isolates from mainland (Daejeon) Korea . All isolates belonged to the BR group and infected both Chinese cabbage and radish. The new TuMV isolates were named KBJ1, KBJ2, KBJ3, KBJ4 and KBJ5. Full-length cDNAs of each isolate were cloned into the pJY vector downstream of cauliflower mosaic virus 35S and bacteriophage T7 RNA polymerase promoters. Four isolates induced very mild symptoms in Nicotiana benthamiana but KBJ5 induced a hypersensitive response. Symptom difference may result from three amino acid differences uniquely present in KBJ5; Gly(381)Asp, Ile(891)Val, and Lys(2522)Glu in P1, P3, and NIb, respectively.