Author
FENG, GUANGYAN - SICHUAN AGRICULTURAL UNIVERSITY | |
XU, LEI - SICHUAN AGRICULTURAL UNIVERSITY | |
WANG, JIANPING - UNIVERSITY OF FLORIDA | |
NIE, GANG - SICHUAN AGRICULTURAL UNIVERSITY | |
Bushman, Shaun | |
XIE, WENGANG - LANZHOU INSTITUTE OF VETERINARY RESEARCH | |
YAN, HAIDONG - SICHUAN AGRICULTURAL UNIVERSITY | |
YANG, ZHONGFU - VIRGINIA TECH | |
GUAN, HAO - SICHUAN AGRICULTURAL UNIVERSITY | |
HUANG, LINKAI - SICHUAN AGRICULTURAL UNIVERSITY | |
ZHANG, XINQUAN - SICHUAN AGRICULTURAL UNIVERSITY |
Submitted to: BMC Genomics
Publication Type: Peer Reviewed Journal Publication Acceptance Date: 6/24/2018 Publication Date: 10/3/2018 Citation: Feng, G., Xu, L., Wang, J., Nie, G., Bushman, B.S., Xie, W., Yan, H., Yang, Z., Guan, H., Huang, L., Zhang, X. 2018. Integration of small RNAs and transcriptome sequencing uncovers a complex regulatory network during vernalization and heading stages of orchardgrass (Dactylis glomerata L.). BMC Genomics. 19(1). https://doi.org/10.1186/s12864-018-5104-0. DOI: https://doi.org/10.1186/s12864-018-5104-0 Interpretive Summary: Timing of optional flowering depends upon the coordination among seasonal environmental cues. For cool season grasses, such as Dactylis glomerata, vernalization induced by low temperature provides competence to initiate flowering after prolonged cold. We combined analyses of the transcriptome and microRNAs (miRNAs) to generate a comprehensive resource for gene regulation during vernalization and flowering. A total of 3,846 differentially expressed genes and 69 differentially expressed miRNAs were identified across five flowering stages. The miRNA targeted genes were involved in phytohormones, transmembrane transport, and plant morphogenesis in response to vernalization. The expression patterns of differentially expressed genes related to plant hormones, stress responses, energy metabolism, and signal transduction changed significantly in the transition from vegetative to reproductive phases. These analyses have provided a platform for investigating consecutive transcriptional and post-transcriptional regulation of critical phases in D.glomerata and provided insights into the genetic engineering of flowering-control in forage crops. Technical Abstract: Timing of optimal flowering depends upon the coordination among seasonal environmental cues. For cool season grasses, such as Dactylis glomerata, vernalization induced by low temperature provides competence to initiate flowering after prolonged cold. We combined analyses of the transcriptome and microRNAs (miRNAs) to generate a comprehensive resource for gene regulation during vernalization and flowering. A total of 3,846 differentially expressed genes and 69 differentially expressed miRNAs were identified across five flowering stages. The MiRNA targeted genes were involved in phytohormones, transmembrane transport, and plant morphogenesis in response to vernalization. The expression patterns of differentially expresed genes related to plant hormones, stress responses, energy metabolism, and signal transduction changed significantly in the transition from vegetative to reproductive phases. These analyes have provided a platform for investigating consecutive transcriptional and post-transcriptional regulation of critical phases in D. glomerata and provided insights into the genetic engineering of flowering-control in forage crops. |