Genome-wide association study of multiple yield components in a diversity panel of polyploid sugarcane (Saccharum spp.)

Authors: YANG, XIPING - University Of Florida; LUO, ZILIANG - University Of Florida; Todd, James; Sood, Sushma; WANG, JIANPING - University Of Florida

Submitted to: Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 12/18/2019
Publication Date: 3/20/2020
Citation: Yang, X., Luo, Z., Todd, J.R., Sood, S.G., Wang, J. 2020. Genome-wide association study of multiple yield components in a diversity panel of polyploid sugarcane (Saccharum spp.). Genetics. Plant Genome 13(1). https://doi.org/10.1002/tpg2.20006.
DOI: https://doi.org/10.1002/tpg2.20006

Interpretive Summary: A diversity panel consisted of 308 accessions was assembled. Nine yield components were evaluated in the diversity panel in three replicates. We deeply sequenced the coding regions of the panel, and identified large amounts of sequence variants with a confident dosage resolution. We performed Genome-wide association study with marker dosages and gene actions taken into consideration. In addition, for the first time, we investigated runs of homozygosity (ROHs), indicators of inbreeding, in this polyploid species, and found that ROHs were associated with total fresh weight and dry weight. The new methods and concepts in this study shed light on research in sugarcane and polyploid species.

Technical Abstract: Sugarcane (Saccharum spp.) is an important economic crop, contributes up to 80% of sugar and approximately 60% bio-fuel globally. To meet the increased demand for sugar and bio-fuel supplies, it is critical to breed sugarcane cultivars with robust performance in yield components. Therefore, dissection of causal DNA sequence variants is of great importance by providing genetic resources and fundamental information for crop improvement. In this study, we evaluated and analyzed nine yield components in a sugarcane diversity panel consisting of 308 accessions primarily selected from the “world collection of sugarcane and related grasses”. By genotyping the diversity panel using target enrichment sequencing, we identified a large number of sequence variants. Genome-wide association study between the markers and traits were conducted with dosages and gene actions taken into consideration. In total, 217 non-redundant markers and 225 candidate genes were identified to be significantly associated with the yield components, which can serve as a comprehensive genetic resource database for future gene identification, characterization, and selection for sugarcane improvement. We further investigated runs of homozygosity (ROH) in the sugarcane diversity panel. We characterized 282 ROHs, and found that the occurrence of ROH in the genome were non-random and probably under selection. ROHs were associated with total fresh weight and dry weight, and high ROHs resulted in decrease of the two traits. This study approved that genomic inbreeding has led to negative impacts on sugarcane yield.