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ARS Home » Southeast Area » Canal Point, Florida » Sugarcane Field Station » Research » Publications at this Location » Publication #353163

Research Project: Identification of Resistant Germplasm and Markers Associated with Resistance to Major Diseases of Sugarcane

Location: Sugarcane Field Station

Title: Sugarcane mosaic virus infects at least five different hosts in South Florida

Author
item Hincapie, M - University Of Florida
item Wei, C - Guangxi University
item Sood, Sushma
item Mollov, Dimitre
item Rott, Philippe - University Of Florida

Submitted to: American Society of Sugar Cane Technologists
Publication Type: Abstract Only
Publication Acceptance Date: 4/30/2018
Publication Date: 6/1/2018
Citation: Hincapie, M., Wei, C., Sood, S.G., Mollov, D.S., Rott, P.C. 2018. Sugarcane mosaic virus infects at least five different hosts in South Florida. American Society of Sugar Cane Technologists. 38, pg.69.

Interpretive Summary: N/A

Technical Abstract: Sugarcane mosaic virus (SCMV) is well known to infect several plant species of the Poaceae family, including cultivated plants and various wild grasses. Patterns of contrasting shades of green were recently observed on leaves of five plant species in South Florida: Columbus grass (Sorghum almum), maize (Zea mays), sorghum (Sorghum bicolor), St. Augustine grass (Stenotaphrum secundatum), and sugarcane (Saccharum spp.). Thirty two plants with these mosaic looking symptoms were collected and tested by reverse-transcription polymerase chain reaction (RT-PCR) using Poaceae potyvirus specific primers (oligo 1n and oligo 2n). All plants produced an amplicon of about 320-330 bp that was sequenced. Amplicons showed highest nucleotide identity with SCMV isolates in GenBank (BLASTn), thus confirming that Columbus grass, maize, sorghum, St Augustine grass, and sugarcane were hosts of the same virus species in South Florida. The genetic relationship between the virus isolates occurring in these five plant hosts is currently being investigated.