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ARS Home » Pacific West Area » Pullman, Washington » Plant Germplasm Introduction and Testing Research » Research » Publications at this Location » Publication #351476

Research Project: Management of Priority Legume, Oilseed, Vegetable, Forage Grass, Sugar, Ornamental, and Medicinal Plant Genetic Resources and Associated Information

Location: Plant Germplasm Introduction and Testing Research

Title: KASP assays for powdery mildew resistance breeding in pea

item Coyne, Clarice - Clare
item MA, YU - Washington State University
item SMITCHGER, JAMIN - Washington State University
item MAIN, DOREEN - Washington State University
item McGee, Rebecca

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 1/15/2018
Publication Date: N/A
Citation: N/A

Interpretive Summary: none

Technical Abstract: Powdery mildew of pea, caused by Erysiphe pisi DC, is a serious production constraint to pea (Pisum sativum L.) production in the U.S. and elsewhere. Utilization of genetic resistance to powdery mildew using er1 has been an effective strategy to manage this disease. This gene, er1, conferring powdery mildew resistance was previously cloned and sequenced, and eight kompetitive allele-specific PCR (KASP) functional markers for each resistance allele were developed. In order to identify additional pea germplasm with powdery mildew resistance, these KASP markers were used to genotype a pea collection derived from the USDA pea single-plant (PSP) collection, USDA advanced breeding lines and a set of U.S. pea cultivars. The efficacy of the KASP markers and the previously reported resistance alleles will be discussed.