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ARS Home » Pacific West Area » Pullman, Washington » Plant Germplasm Introduction and Testing Research » Research » Publications at this Location » Publication #351319

Research Project: Management of Priority Legume, Oilseed, Vegetable, Forage Grass, Sugar, Ornamental, and Medicinal Plant Genetic Resources and Associated Information

Location: Plant Germplasm Introduction and Testing Research

Title: Genome wide association studies on yield components using a lentil genetic diversity panel

Author
item SMITCHGER, JAMIN - Washington State University
item MA, YU - Washington State University
item ZHENG, PING - Washington State University
item Coyne, Clarice - Clare
item McGee, Rebecca
item MAIN, DOREEN - Washington State University

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 2/22/2018
Publication Date: N/A
Citation: N/A

Interpretive Summary: none

Technical Abstract: The cool season food legume research community are now at the threshold of deploying the cutting-edge molecular genetics and genomics tools that have led to significant and rapid expansion of gene discovery, knowledge of gene function (including tolerance to biotic and abiotic stresses) and genetic improvement of many crop species. We employed two-enzyme genotyping-by-sequencing to genotype a 367 accession lentil diversity panel (USDA Lentil Core Collection) for use in genome wide association studies for yield components. The SNP markers were filtered for a minimum site count of 75% and minimum allele frequency of 0.05 resulting in 4,084 total SNPs in the final data set. We will present preliminary SNP marker discovery based on one year of field phenotyping for days to 50% flowering, plant height, height to lowest pod, average seed per pod, pod dehiscence, 90% maturity and seed yield per plant.