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ARS Home » Pacific West Area » Pullman, Washington » Plant Germplasm Introduction and Testing Research » Research » Publications at this Location » Publication #347405

Research Project: Management of Plant Genetic Resources and Associated Information

Location: Plant Germplasm Introduction and Testing Research

Title: Identification of quantitative trait loci (QTL) controlling cold tolerance in chickpea recombinant RIL population (CRIL2) from Cicer arietinum L. x Cicer reticulatum

Author
item Mugabe, Deus - Washington State University
item Ma, Yu - Washington State University
item Coyne, Clarice - Clare
item Mcgee, Rebecca

Submitted to: North American Pulse Improvement Association
Publication Type: Abstract Only
Publication Acceptance Date: 10/1/2017
Publication Date: N/A
Citation: N/A

Interpretive Summary: None.

Technical Abstract: Published yields for chickpea (Cicer arietinum L.) are higher when the crop is planted in the fall rather than in the spring seasons (Singh et al 1989, Singh et al 1997). Because of its lack of cold hardiness alleles to survive freezing temperatures, chickpea is planted in the spring in temperate regions which leads to disadvantages like reduced yield and inadequate crop residue for soil cover. This project focuses on understanding cold tolerance in chickpea. The objectives are to confirm the cold tolerance of the CRIL2 population under field and controlled conditions, construct libraries to genotype the population using genotyping-by-sequencing, and identify the genetic determinates using both QTL and comparative mapping analyses. The CRIL2 seed used in the experiment were F6 recombinant inbred lines. The population is made up of 129 RILs that were derived from the cross of a domesticated desi type Cicer arietinum L.(ICC 4958) and Cicer reticulatum (PI 489777), a wild type parent that has shown good levels of cold tolerance, cross number; X92C031. The experiments were set up in two locations with different winter conditions; Central Ferry, WA, 46°39’5.1” N; 117°45’45.4” W and Washington State University’s Spillman farm in Pullman, WA, 46°44’3.2’’ N; 117°7’25.8” W, using a randomized complete block (RCB) experimental design. Assessment of cold tolerance was based on mean stand counts. and plant leaf damage. Stand count data was collected before and after the winter season 2016-2017, and plant damage visual rating was done for both field environments after the winter, March 2017. A freezing experiment using a growth chamber is also underway for additional controlled environmental data. For genotypic data, CRIL2 libraries were constructed and were genotyped using genotyping by sequencing (GBS) technique. The genotypic and phenotypic data from all the environments in the study will be used in QTL analysis to locate the regions controlling cold tolerance in CRIL2. Further phenotypic trait data such as; leaf structure, growth habit, height, and flowering days was also be recorded.