Draft genome sequence analysis of eight streptogramin-resistant Enterococcus species isolated from animal and environmental sources in the US

Authors: SHARMA, POONAM - Orise Fellow; GUPTA, SUSHIM - Orise Fellow; Barrett, John; Hiott, Lari; House, Sandra; Woodley, Tiffanie; Frye, Jonathan; Jackson, Charlene

Submitted to: Genome Announcements
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 10/17/2017
Publication Date: 11/1/2017
Citation: Sharma, P., Gupta, S., Barrett, J.B., Hiott, L.M., House, S.L., Woodley, T.A., Frye, J.G., Jackson, C.R. 2017. Draft genome sequence analysis of eight streptogramin-resistant Enterococcus species isolated from animal and environmental sources in the US. Genome Announcements. 16;5(46). pii: e01287-17. doi: 10.1128/genomeA.01287-17.
DOI: https://doi.org/10.1128/genomeA.01287-17

Interpretive Summary: The streptogramin antimicrobials have been used in both human and veterinary medicine. Quinupristin/Dalfopristin is used in human medicine to treat vancomycin-resistant Enterococcus faecium bacteremia while virginiamycin was used in food animal production for decades. Streptogramin resistance in enterococci from both humans and animals has been documented and many mechanisms of resistance have been described. In this study, nine streptogramin-resistant enterococci from animals and the environment were sequenced. Antimicrobial resistance genes were identified conferring resistance to the macrolide-lincosamide-streptogramins, aminoglycosides, tetracyclines, beta-lactams, and glycopeptides. The routine collection and analysis of these animal and environmental associated bacteria will help improve monitoring and surveillance of antimicrobial resistance and identify novel mechanism of streptogramin resistance. This information will be useful for policy makers and researchers when studying the effects of antimicrobials used in animals on resistance in humans and the environment.

Technical Abstract: Here, we present the draft genome sequences of eight streptogramin-resistant Enterococcus spp. (n=8) isolated from animals and an environmental source in the US from 2001-2004. Antimicrobial resistance genes were identified conferring resistance to the macrolide-lincosamide-streptogramins, aminoglycosides, tetracyclines, beta-lactams, and glycopeptides.