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ARS Home » Midwest Area » West Lafayette, Indiana » Crop Production and Pest Control Research » Research » Publications at this Location » Publication #347136

Research Project: Identification, Characterization, and Deployment of Genes Important during Seed Development in Legumes

Location: Crop Production and Pest Control Research

Title: A novel dsRNA virus stimulates sporulation of Phytophthora infestans and may have contributed to late blight epidemics

Author
item Cai, Guohong
item MYERS, KEVIN - Cornell University - New York
item FRY, WILLIAM - Cornell University - New York
item HILLMAN, BRADLEY - Rutgers University

Submitted to: International Congress of Plant Pathology Abstracts and Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 3/27/2018
Publication Date: 7/29/2018
Citation: Cai, G., Myers, K., Fry, W.E., Hillman, B.I. 2018. A novel dsRNA virus stimulates sporulation of Phytophthora infestans and may have contributed to late blight epidemics. International Congress of Plant Pathology Abstracts and Proceedings.596-P.

Interpretive Summary:

Technical Abstract: Phytophthora infestans is the causal agent of potato and tomato late blight. To identify genetic elements contributing to this disease, several dsRNA viruses have been characterized in this organism. One of the viruses, Phytophthora infestans RNA virus 2 (PiRV-2), is 11,170bp in length and without a polyA tail. It contains a large open reading frame (ORF) with short 5’- and 3’- untranslated regions. The ORF is predicted to encode a polyprotein of 3710 aa (calculated molecular weight 410.94 kDa). This virus lacks significant similarity to any known viruses, even in the RNA-dependent RNA polymerase region. Phylogenetic analysis showed it should be classified into a new virus family. Comparing isogenic strains with or without the virus demonstrated that the virus stimulated sporangia production multiple folds. This virus was faithfully transmitted through asexual reproduction and was found in most strains in the US-8 and US-22 lineages. It may have contributed to the success of these two lineages in recent late blight epidemics. This is the first report that a potential hypervirulent virus may contribute to late blight epidemics and presents a novel target for late blight control.