Location: Fruit and Tree Nut ResearchTitle: Characterization of microsatellites from a genome of Venturia carpophila
|BRANNEN, PHILLIP - University Of Georgia|
|ADASKAVEG, JAMES - University Of Georgia|
Submitted to: American Phytopathological Society Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 5/17/2017
Publication Date: 12/1/2017
Citation: Chen, C., Brannen, P.M., Adaskaveg, J.E., Bock, C.H. 2017. Characterization of microsatellites from a genome of Venturia carpophila. American Phytopathological Society Annual Meeting. 107:S5.124.
Technical Abstract: Microsatellites were mined from a genome of Venturia carpophila and 192 were selected to screen 39 isolates of the fungus collected from peach and nectarine in the southeastern USA. Reliable, polymorphic amplicons were produced by 32 of the selected primers. A preliminary analysis of the genetic diversity of the panel of isolates was performed. Allele number ranged from 2 to 9, and the polymorphic information content from 0.097 to 0.792. Shannon’s information index was 0.914, indicating substantial genetic diversity. Stoddart and Taylor’s index of diversity and Simpson’s index also indicated high diversity (32.4 and 0.969, respectively). Evenness within the sample was high (0.955), but there was strong evidence for haploid linkage disequilibrium (3.799 (P=0.001)). Observations on diversity were supported by the dissimilarity analysis, which showed little affinity for clustering based on isolate source population, location or host. The microsatellites developed in this study should be useful in future research of the population genetic structure and dynamics of V. carpophila, and evaluating the risks posed by the ability of the pathogen to adapt on peach and possibly other stone fruit hosts in the USA and elsewhere in the world.