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Research Project: Intervention Strategies to Control Endemic and New and Emerging Viral Diseases of Swine

Location: Virus and Prion Research

Title: Comparison of the transcriptome response within the tracheobronchial lymphnode following infection with PRRSV, PCV2 or SIV

item Miller, Laura
item FLEMING, DAMARIUS - Orise Fellow
item Harhay, Gregory
item Bayles, Darrell
item Kehrli Jr, Marcus
item Lager, Kelly

Submitted to: Research Workers in Animal Diseases Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 9/19/2017
Publication Date: 12/3/2017
Citation: Miller, L.C., Fleming, D., Harhay, G., Bayles, D., Kehrli, M., Lager, K. 2017. Comparison of the transcriptome response within the tracheobronchial lymphnode following infection with PRRSV, PCV2, or SIV. 98th Conference of Research Workers in Animal Diseases. Abstract No. 190, p. 107.

Interpretive Summary:

Technical Abstract: Porcine reproductive and respiratory syndrome virus (PRRSV) is a major respiratory pathogen of swine that has become extremely costly to the swine industry worldwide, often causing losses in production and animal life due to their ease of spread. However, the intracellular changes that occur in pigs following viral respiratory infections are still scantily understood for PRRSV, as well as, other viral respiratory infections. The aim of this study was to acquire a better understanding of PRRS disease by comparing gene expression changes that occur in tracheobronchial lymph nodes of pigs infected with either porcine reproductive and respiratory syndrome virus (PRRSV), porcine circovirus type 2 (PCV2), or swine influenza virus (SIV) infections. The study identified and compared gene expression changes in the TBLN of 16 pigs following infection by PRRSV, PCV2, SIV, or sham inoculation. Total RNA was pooled for each group and time-point (1, 3, 6, and 14 DPI) to make 16 libraries, for analysis by Digital Gene Expression Tag Profiling (DGETP). The data underwent standard filtering to generate a list of sequence tag raw counts that were then analyzed using multidimensional and differential expression statistical tests. The results showed that PRRSV, SIV and PCV-2 viral infections followed a clinical course in the pigs typical of experimental infection of young pigs with these viruses. Gene expression results echoed this course, as well as, uncovered genes related to shared and unique host immune responses to the 3 viruses. By testing and observing the host response to other respiratory viruses, our study has elucidated similarities and differences that can assist in development of vaccines and therapeutics that shorten or prevent a chronic PRRSV infection.