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ARS Home » Southeast Area » Mayaguez, Puerto Rico » Tropical Crops and Germplasm Research » Research » Publications at this Location » Publication #341668

Research Project: Genetic Enhancement of Common Bean Using Exotic Germplasm for Biotic and Abiotic Stress Tolerance

Location: Tropical Crops and Germplasm Research

Title: Selection of bean lines that combine resistance to web blight and common bacterial blight

Author
item Martinez-figueroa, H. - University Of Puerto Rico
item Rosas, Juan - Escuela Agricola Panamericana
item Estevez De Jensen, Consuelo - University Of Puerto Rico
item Porch, Timothy - Tim
item Beaver, James - University Of Puerto Rico

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 4/30/2017
Publication Date: N/A
Citation: N/A

Interpretive Summary: Web blight causes significant reductions in the yield and quality of common beans produced in the humid, lowland tropics. A total of 644 lines from different breeding programs were evaluated for reaction to web blight and other diseases using conventional plant breeding techniques and marker-assisted selection (MAS). Field trials were conducted over two years at Isabela, Puerto Rico to evaluate the reaction of the bean lines to web blight. The field trials were planted using RCB designs. Web blight resistance was identified in 37 lines that had mean scores = 4.5 on the CIAT disease rating scale during both growing seasons. Some lines (TARS MST1, PR1147-1 and Amadeus 77) produced mean seed yields > 1,800 kg/ha. Some lines with high seed yield potential had a low percentage of damaged seed despite having presented a high degree of damage in the foliage. It is advisable to conduct evaluations for both leaf reaction and seed damage to identify lines with resistance to web blight. Evaluations for reaction to common bacterial blight with two strains of the pathogen were conducted in a greenhouse. Lines with high levels of CBB resistance were identified. Greenhouse inoculations with the bean common mosaic virus (BCMV) strain NL3 were conducted. MAS was used to detect genes conferring resistance to BCMV, bean common mosaic necrosis virus and bean golden yellow mosaic virus. Twelve lines were identified with multiple disease resistance. These lines also had mean seed yields > 1,400 kg/ha during the second growing season and produced high quality seed. A few of the most promising white, black and small red lines of bean from this study will be considered for release as commercial varieties or improved breeding lines.

Technical Abstract: Web blight caused by Thanatephorus cucumeris Frank (Donk) causes significant reductions in the yield and quality of beans produced in the humid, lowland tropics. A total of 644 lines from different breeding programs were evaluated for reaction to web blight and other diseases using conventional plant breeding techniques and marker-assisted selection (MAS). Field trials were conducted over two years at Isabela, Puerto Rico to evaluate the reaction of the bean lines to web blight. The field trials were planted using RCB designs. Web blight resistance was identified in 37 lines that had mean scores = 4.5 on the CIAT disease rating scale during both growing seasons. Some lines (TARS MST1, PR1147-1 and Amadeus 77) produced mean seed yields > 1,800 kg/ha. Some lines with high seed yield potential had a low percentage of damaged seed despite having presented a high degree of damage in the foliage. It is advisable to conduct evaluations for both leaf reaction and seed damage to identify lines with resistance to web blight. Evaluations for reaction to common bacterial blight with two strains of Xap, 484A and UPR 3353, were conducted in a greenhouse. Lines with high levels of CBB resistance were identified. Greenhouse inoculations with the BCMNV strain NL3 were conducted. MAS was used to detect genes conferring resistance to BCMV, BCMNV and BGYMV. Twelve lines were identified with multiple disease resistance. These lines also had mean seed yields > 1,400 kg/ha during the second growing season and produced high quality seed. A few of the most promising white, black and small red lines of bean from this study will be considered for release as commercial varieties or improved breeding lines.