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ARS Home » Southeast Area » Mayaguez, Puerto Rico » Tropical Crops and Germplasm Research » Research » Publications at this Location » Publication #341556

Research Project: Genetic Enhancement of Common Bean Using Exotic Germplasm for Biotic and Abiotic Stress Tolerance

Location: Tropical Crops and Germplasm Research

Title: Identification of QTL in a tepary bean RIL population under abiotic stress

Author
item Rodriguez, Iveth - University Of Puerto Rico
item Beaver, James - University Of Puerto Rico
item Hart, John
item Porch, Timothy - Tim

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 4/28/2017
Publication Date: N/A
Citation: N/A

Interpretive Summary: High temperatures and drought are critical abiotic factors that limit the production of grain legumes, especially in tropical countries. Tepary bean is a species that is tolerant to high temperatures and drought. It is also closely related to common bean. The objective of the study was to identify quantitative trait loci (QTL) in a recombinant inbred line population (RIL) of tepary bean in trials conducted under terminal drought and high temperature conditions. To identify QTL, the RIL population was exposed to terminal drought stress in one trial and to high-temperature stress in another trial in 2015 and 2016, respectively. Both experiments were carried out at the Agricultural Experiment Station of the University of Puerto Rico in Juana Díaz, Puerto Rico. The characteristics evaluated in the field were: vigor, days at flowering, days to maturity, canopy temperature, chlorophyll content, plant height, vegetation indices, pollen release, and yield components. Under stress from terminal drought and high temperatures in the field, there were significant differences in the evaluated characteristics (p=0.05). The linkage map of the RIL population was constructed using ~ 1300 SNPs with the common bean genome as a reference. We identified QTLs associated with agronomic, phenological and seed characteristics on chromosomes 4, 7 and 8. The results of this study will improve our understanding of the genetics of tepary bean and the mechanisms of tolerance to abiotic factors. The results will also be useful for the improvement of common bean and tepary bean for abiotic stress.

Technical Abstract: High temperatures and drought are critical abiotic factors that limit the production of grain legumes, especially in tropical countries. Tepary bean (Phaseolus acutifolius A. Gray) is a species that is tolerant to high temperatures and drought. It is also closely related to common bean (Phaseolus vulgaris L.). The objective of the study was to identify quantitative trait loci (QTL) in a recombinant inbred line population (RIL) of tepary bean under in trials conducted under terminal drought and high temperature conditions. To identify QTL, the RIL population was exposed to terminal drought stress in one trial and to high-temperature stress in another trial in 2015 and 2016, respectively. Both experiments were carried out at the Agricultural Experiment Station of the University of Puerto Rico in Juana Díaz, Puerto Rico. The characteristics evaluated in the field were: vigor, days at flowering, days to maturity, canopy temperature, chlorophyll content, plant height, vegetation indices, pollen release, and yield components. Under stress from terminal drought and high temperatures in the field, there were significant differences in the evaluated characteristics (p=0.05). The linkage map of the RIL population was constructed using ~ 1300 SNPs with the common bean genome as a reference. We identified QTLs associated with agronomic, phenological and seed characteristics on chromosomes 4, 7 and 8. The results of this study will improve our understanding of the genetics of tepary bean and the mechanisms of tolerance to abiotic factors. The results will also be useful for the improvement of common bean and tepary bean for abiotic stress.