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ARS Home » Midwest Area » Ames, Iowa » Corn Insects and Crop Genetics Research » Research » Publications at this Location » Publication #340826

Research Project: Managing Insects in the Corn Agro-Ecosystem

Location: Corn Insects and Crop Genetics Research

Title: The complete mitochondrial genome of Anoplocnemis curvipes F. (Coreinea, Coreidae, Heteroptera), a pest of fresh cowpea pods

Author
item Valero, Carmen - University Of Illinois
item Ojo, James - Kwara State University
item Sun, Weilin - Michigan State University
item Tamo, Manuele - International Institute Of Tropical Agriculture (IITA)
item Coates, Brad
item Pittendrigh, Barry - Michigan State University

Submitted to: Mitochondrial DNA Part B
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 6/24/2017
Publication Date: 7/18/2017
Citation: Valero, C.M., Ojo, J.A., Sun, W., Tamo, M., Coates, B.S., Pittendrigh, B.R. 2017. The complete mitochondrial genome of Anoplocnemis curvipes F. (Coreinea, Coreidae, Heteroptera), a pest of fresh cowpea pods. Mitochondrial DNA Part B. 2(2):421-423. https://doi.org/10.1080/23802359.2017.1347829.
DOI: https://doi.org/10.1080/23802359.2017.1347829

Interpretive Summary: Pest insects feed upon cultivated crops worldwide and thereby cause severe plant damage that reduces yield, grain quality, and producer profitability. Insects that feed on the pods of legume seed crops by sucking phloem are also capable of transmitting plant disease causing viruses. Many of these insect pest species are not well described, which leads to difficulties in differentiating from related species as well as designing effective management practices. This research generated by an ARS researcher in conjunction with an international team of collaborators provides basal genetic information on a pod sucking insect of cultivated beans and peas, called the giant coreid bug, by generating a mitochondral genome sequence. The sequence data was applied to determine the relationship of the species towards other insects as well as differentiate it genetically. These findings demonstrate the ability of next generation sequencing to allow rapidly acquire genetic information that has application in agricultural insect pest control. This research is applicable to work by university, government and private industry stakeholders that aim to develop sustainable integrated pest management practices.

Technical Abstract: The complete 16,345-bp mitochondrial genome of the agriculturally-destructive pod sucking pest, the giant coreid bug, Anoplocnemis curvipes (Hemiptera: Coreidae), was assembled from paired end next generation sequencing reads. The A. curvipes mitochondrial genome consists of 13 protein coding genes (PCGs), 22 tRNAs, 2 rRNAs and a control region in the order and orientation typical among insects. PCGs initiation codons (ATG, ATC, ATT and ATA) with termination codon (TAA) are used with the exception of TAG stop codons by Cytb and ND3. All tRNA genes fold into predicted clover-leaf secondary structures having requisite triplets on the anticodon loop, apart from tRNA-Ser1 (AGN) whose dihydrouridine (DHU) arm forms a simple loop. The phylogenetic analysis of hemipteran mitogenomes cluster to the family level and support the monophyly of the five superfamilies in Pentatomomorpha of Hemiptera. The Coreoidea and Pyrrhocoroidea are sister groups, while Coreidae and Alydidae are sister groups to Rhopalidae. These analyses provide insight to mitogenomics and evolutionary relationships among pentatomoid insects.