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ARS Home » Midwest Area » Madison, Wisconsin » U.S. Dairy Forage Research Center » Cell Wall Biology and Utilization Research » Research » Publications at this Location » Publication #338699

Title: Effects of xanthosine on gene expression of mammary epithelial cells using RNA sequencing of goat milk fat globules

item CHOUDHARY, SHANTI - Punjab Agricultural University
item CHOUDHARY, RATAN - Punjab Agricultural University
item RAMNEEK, VERMA - Punjab Agricultural University
item SETHI, R - Punjab Agricultural University
item MUKHOPADHYAY, C - Punjab Agricultural University
item Bickhart, Derek
item Li, Wenli
item Capuco, Anthony

Submitted to: American Dairy Science Association Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 3/14/2017
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Although intramammary xanthosine (XS) treatment was reported to increase the mammary stem cell population and milk yield in bovine and caprine, underlying molecular mechanisms remain unclear. The goal of this study was to evaluate effects of XS treatment on the mammary transcriptome in early lactation dairy goats. Primiparous Beetle goats were used. Five d after kidding, one gland was infused with XS (treatment [TRT]) twice daily for 3 d, and the other gland served as control (CON). Milk from TRT and CON glands was collected 10 d after the last treatment, followed by mammary epithelial cells (MEC) isolation and RNA extraction. We characterized XS-induced transcriptome changes in MEC of 2 goats, using RNA sequencing technology. Processed reads were aligned to the goat genome using STAR. Transcript raw read counts were obtained using HTseq. Differential transcript analysis was performed using cuffdiff and DESeq2. Differentially expressed genes ([DEGs]; P < 0.05) were used to identify gene ontology terms (PANTHER) and gene networks (STRING db). Response to XS treatment was based on identification of DEGs between TRT and CON glands of each goat. Pathways impacted by these DEGs include cell communication, cell cycle, and cell proliferation. A predominant portion of the DEGs showed down-regulation by XS treatment. Functional prediction of these genes include: (i) regulation of inflammatory and immune response (TLR4, IL8, CDKN1A (p21), S100A8, S100A12, VIM) and (ii) anti-proliferative signals by dual specificity phosphatases (DUSP). Remaining DEGs reflected up-regulation by treatment. Predicted function of these genes include: (i) mRNA processing and splicing, and (ii) signaling pathways. Notably, up-regulation of RHOA highlighted its potential role in modulating response to XS through: (1) linking plasma membrane receptors, (2) involvement of focal adhesion, and (3) regulating cellular response to stimulus. This study documented XS-induced transcriptome changes in milk producing cells of goats and suggested that XS treatment is likely associated with molecular mechanisms of enhanced cell proliferation in milk producing cells in goats.