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Title: Metabolic fate of strawberry polyphenols after chronic supplementation in healthy older adults

item SANDHU, AMANDEEP - Illinois Institute Of Technology
item Miller, Marshall
item Thangthaeng, Nopporn
item SCOTT, TAMMY - Jean Mayer Human Nutrition Research Center On Aging At Tufts University
item Shukitt-Hale, Barbara
item EDIRISINGHE, INDIKA - Illinois Institute Of Technology
item BURTON-FREEMAN, BRITT - Illinois Institute Of Technology

Submitted to: Berry Health Benefits Symposium
Publication Type: Abstract Only
Publication Acceptance Date: 2/10/2017
Publication Date: 3/28/2017
Citation: Sandhu, A., Miller, M.G., Thangthaeng, N., Scott, T.M., Shukitt Hale, B., Edirisinghe, I., Burton-Freeman, B. 2017. Metabolic fate of strawberry polyphenols after chronic supplementation in healthy older adults [abstract]. 2017 Berry Health Benefits Symposium Pre-Proceedings, 2017 Berry Health Benefits Symposium, March 28-30, 2017, Pismo Beach, California. p. 86.

Interpretive Summary:

Technical Abstract: Strawberries are considered as a functional fruit due to the presence of a wide array of nutrients including polyphenols such as anthocyanins, procyanidins and ellagitannins. These polyphenols are absorbed and metabolized to various phenolic metabolites/conjugates in the body which may play an active role in disease prevention or protection. In the present study, we explored the metabolic fate of strawberry polyphenols after chronic (90 days) supplementation of freeze-dried strawberry (24 g/d, equivalent to 1 cup of fresh strawberries) in 18 healthy older adults (66.7 ± 4.4 y). Blood samples were collected at baseline (t=0 h) and 2 h post consumption on day 1 (no treatment), day 45 and day 90. A pooled plasma sample (t=0 and 2 h) from six randomly selected subjects was used to identify the phenolic metabolites/conjugates using ultra high-performance liquid chromatography coupled with electrospray ionization triple quadrupole mass spectrometer. Parent anthocyanins and their conjugated metabolites were identified including cyanidin-3-O-glucoside, pelargonidin-3-O-rutinoside, pelargonidin-3-O-glucoside, pelargonidin glucuronide and pelargonidin sulfate. We were able to identify urolithin A glucuronide in the plasma samples for the first time after strawberry consumption. A total of 18 phenolic acid metabolites (glucuronides and sulfates) were identified. Hippuric acid sulfate, vanillic acid glucuronide, isovanillic acid glucuronide, 2, 3-dihydroxybenzoic acid and p-coumaric acid were observed only in 2 h plasma samples compared to 0 h samples. Our results suggest that strawberry polyphenols are absorbed and extensively metabolized resulting in the production of various phenolic acid derivatives and their conjugates, all together contributing to the bioavailability and beneficial effects associated with strawberry consumption. A complete set of quantitative data will be presented at the meeting.