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ARS Home » Southeast Area » Miami, Florida » Subtropical Horticulture Research » Research » Publications at this Location » Publication #336947
Research Project: Methyl Bromide Replacement: Mitigation of the Invasive Pest Threat from the American Tropics and Subtropics

Location: Subtropical Horticulture Research

Title: Characterization of volatile and polar compounds of Jiaogulan Tea [Gynostemma pentaphyllum (Thunb.) Makino] by hyphenated analytical techniques

Author
item BLYTHE, EUGENE - Mississippi State University
item DEMIRCI, BETUL - Anadolu Universtiy
item FATIH, GOGER - Anadolu Universtiy
item Tabanca, Nurhayat

Submitted to: Asian Journal of Chemistry
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 2/9/2017
Publication Date: 4/10/2017
Citation: Blythe, E.K., Demirci, B., Fatih, G., Tabanca, N. 2017. Characterization of volatile and polar compounds of Jiaogulan Tea [Gynostemma pentaphyllum (Thunb.) Makino] by hyphenated analytical techniques. Asian Journal of Chemistry. 29:1285-1290. doi:10.14233/ajchem.2017.20467.

Interpretive Summary: Gynostemma pentaphyllum Makino is a Chinese medicinal herb which grows widely in southern China, Japan, India, and Korea. In China, G. pentaphyllum is called jiaogulan and is often used as a herbal tea. G. pentaphyllum has been reported to have significant bioactivity in treatment of a wide range of diseases, including various types of cancer and type 2 diabetes. In this study, we investigated volatile composition of jiaogulan tea using headspace-solid phase microextraction (HS-SPME) coupled with gas chromatography-mass spectrometry and found benzaldehyde, 1,8-cineole, 6-methyl-5-hepten-2-one, (Z)-3-hexenal, and a-ionone. Methanol and water extracts from the G. pentaphyllum leaves were also studied by using LC-ESI-MS/MS systems. Chlorogenic acids, flavonoids, and gypenosides were identified. Methanol and water extracts were examined for their antioxidant activity using DPPH, ß-carotene/linoleic acid, and ABTS radical scavenging assays. Weak antioxidant activity was observed.

Technical Abstract: Jiaogulan [Gynostemma pentaphyllum (Thunb.) Makino] is a Chinese medical plant from southern Asia that has rapidly gained popularity and interest for its health-promotive and therapeutic properties. The volatile composition of jiaogulan tea was analyzed by using headspace-solid phase microextraction (HS-SPME) coupled with gas chromatography-mass spectrometry (GC-MS). A total of 29 volatile components were detected in the jiaogulan tea, with major compounds being benzaldehyde (15.3%), 1,8-cineole (8.7%), 6-methyl-5-hepten-2-one (7.8%), (Z)-3-hexenal (7.6%) and a-ionone (6.3%). The chemical characterization of MeOH extract and 5% infusion from the G. pentaphyllum leaves was determined by using LC-ESI-MS/MS systems. Chlorogenic acids, flavonoids and gypenosides were determined. Compounds quercetin 3-O-hexoside, isorhamnetin rutinoside, and kaempferol dimethyl ether were identified in the extracts for the first time. Phenolic content was determined by gallic acid equivalents and antioxidant activity of the extracts was evaluated by DPPH, ß-carotene/linoleic acid, and ABTS radical scavenging assays. Both extracts showed weak antioxidant activity.