Submitted to: Journal of Federation of American Societies for Experimental Biology
Publication Type: Abstract Only
Publication Acceptance Date: 12/1/2016
Publication Date: 4/1/2017
Citation: Yan, L., Sundaram, S. 2017. Effects of voluntary running with defined distances on body adiposity and its associated inflammation in mice fed a high-fat diet [abstract]. Federation of American Societies for Experimental Biology Conference. 31:794.7.
Technical Abstract: Sedentary lifestyle contributes to obesity. This study examined the effect of quantitative voluntary running on body adiposity and its associated inflammation in mice fed a high-fat diet. Male C57BL/6 mice were assigned into six groups and fed the AIN93G (sedentary) or a high-fat diet (sedentary, unrestricted running, or with 25%, 50%, or 75% restriction in running activity) for 12 weeks. The average daily running distance was 8.3, 6.3, 4.2, and 2.1 km for the unrestricted, 25%, 50%, and 75% restricted runners, respectively. The high-fat diet increased body fat mass by 46% compared to the AIN93G diet in sedentary mice. Running reduced the fat mass in a dose dependent manner; there was no difference between sedentariness and running at 2.1 km/d. The high-fat diet significantly increased blood glucose, plasma insulin, HOMA-IR, and plasma and adipose concentrations of leptin and monocyte chemotactic protein-1 (MCP-1) in sedentary mice. Running, despite continued consumption of the high-diet, reduced these variables in dose-dependent manners. Running at 8.3 (unrestricted) and 6.3 km/d (25% restricted) had the greatest but similar reductions in, whereas running at 2.1 km/d did not affect (except MCP-1), these measurements. Running, regardless of daily distance, significantly reduced MCP-1 in both plasma and adipose tissues. In conclusion, voluntary running at 6.3 km/d is optimal to reduce body adiposity and its associated inflammation and metabolic disturbance in C57BL/6 mice. Results that running at 2.1 km/d reduced MCP-1 without affecting body fat mass show the benefits of moderate exercise in improving low-grade inflammation. It suggests that different mechanisms, rather than reducing body adiposity, are responsible for this improvement.