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ARS Home » Midwest Area » St. Paul, Minnesota » Cereal Disease Lab » Research » Publications at this Location » Publication #334433

Research Project: CEREAL RUST FUNGI: GENETICS, POPULATION BIOLOGY, AND HOST-PATHOGEN INTERACTIONS

Location: Cereal Disease Lab

Title: Identification and validation of a major cadmium accumulation locus and closely associated SNP markers in North Dakota durum wheat cultivars

Author
item ABUHAMMAD, WESAM - North Dakota State University
item MAMIDI, SUJAN - North Dakota State University
item KUMAR, AJAY - North Dakota State University
item PIRSEYEDI, SEYED - North Dakota State University
item Kianian, Shahryar
item ALAMRI, MOHAMMED - King Saud University
item MERGOUM, MOHAMED - North Dakota State University
item ELIAS, ELIAS - North Dakota State University

Submitted to: Molecular Breeding
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 7/19/2016
Publication Date: 7/27/2016
Citation: Abuhammad, W.A., Mamidi, S., Kumar, A., Pirseyedi, S., Kianian, S., Alamri, M., Mergoum, M., Elias, E. 2016. Identification and validation of a major cadmium accumulation locus and closely associated SNP markers in North Dakota durum wheat cultivars. Molecular Breeding. 36:112. doi: 10.1007/S11032-016-0536-1.

Interpretive Summary: Durum wheat has the tendency of accumulating more cadmium, a biotoxic heavy metal, in seeds than other commonly grown cereals, thus posing a serious food safety/public health concern. This could have serious negative impact on the national pasta industry and the international export market of durum wheat. Phenotypic analysis for low cadmium content lines is expensive and time consuming. In this study two durum wheat populations were analyzed for cadmium content. A major locus on chromosome arm 5BL was identified explaining a large portion of observed phenotypic variation. The association marker loci were converted into user friendly markers for further assays and they were validated in additional populations and lines. Development of suitable marker assay, associated with major cadmium uptake locus would help in the selection for llines betters suited for the consumers.

Technical Abstract: Durum wheat has the tendency of accumulating more cadmium (Cd), a biotoxic heavy metal, in seeds than other commonly grown cereals, thus posing a serious food safety/public health concern. This could have serious negative impact on the national pasta industry and the international export market of durum wheat. The phenotyping for selecting low Cd lines, is expensive and time consuming. The use of markers could be a more sustainable approach for selecting lines with low Cd levels. Here, a RIL population developed from a cross between Grenora (high Cd) Å~ Haurani (low Cd) and two association mapping panels consisting of advanced breeding lines from the North Dakota durum wheat breeding program, were used to identify QTL and associated markers for Cd. A major QTL, with Haurani contributing low Cd uptake allele and explaining 54.3 % phenotypic variation, was detected on chromosome 5BL.Association mapping using 2010 collection validated the results of linkage mapping and identified major QTL on 5BL. The 2009 collection, showed the presence of a major QTL on chromosome 2B. The SNP marker associated with major QTL on 5BL was converted to user friendly KASPar assay. The major QTL and associated KASPar marker was further validated using another RIL population developed from a cross of Strongfield (low Cd) and Alkabo (high Cd). The development of suitable marker assay, associated with major Cd uptake QTL, would help the selection for low Cd accumulating lines, minimizing the costly phenotypic evaluation for this important trait.