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ARS Home » Southeast Area » Canal Point, Florida » Sugarcane Field Station » Research » Publications at this Location » Publication #331229

Research Project: Enhancement of Sugarcane Germplasm for Development of Stress Tolerant, High Yielding Cultivars

Location: Sugarcane Field Station

Title: Unravel the key genes potentially related to high strength of cotton fiber by comparative phenotypic and genomic analyses

Author
item Islam, Md
item Fang, David
item Kim, Hee-Jin

Submitted to: Atlas of Science
Publication Type: Literature Review
Publication Acceptance Date: 5/30/2016
Publication Date: 5/31/2016
Citation: Islam, M.S., Fang, D.D., Kim, H.J. 2016. Unravel the key genes potentially related to high strength of cotton fiber by comparative phenotypic and genomic analyses. Atlas of Science. Online.

Interpretive Summary: The demand of high strength of cotton fibers has been increased dramatically with the advent of modern high speed spinning technology for producing yarn. Bundle fiber strength is affected by fiber-to-fiber interactions in addition to the individual fiber strength. The bundle fiber strength is not always correlated with the strength measured from individual fibers and molecular mechanisms regulating fiber strength remain unknown. Gossypium hirsutum germplasm near isogenic lines (NILs), MD52ne obtained a gene promoting fiber strength became genetically almost identical to the MD90ne through back cross breeding. The molecular mechanisms behind the formation of superior individual fiber strength were unveiled by using these two NILs. Comparative fiber property analyses revealed that MD52ne have higher (~20%) fiber strength both single and bundle than its NIL MD90ne. Genome mapping analyses using F2 population of these two NILs discovered loci associated with high fiber strength is located on Upland cotton chromosome A03 at 84 mega base pairs. Comparative transcriptome analyses revealed that differential expressions of the genes involved in crystalline cellulose assembly, ethylene phytohormone and receptor-like kinase (RLK) signaling pathways between the MD52ne and MD90ne developing fibers. The superior BFS of MD52ne fibers resulted from high individual fiber strength with minor contributions from greater fiber length and the RLKs is candidate genes for regulating cotton fiber cell wall assembly and strength.

Technical Abstract: The demand of high strength of cotton fibers has been increased dramatically with the advent of modern high speed spinning technology for producing yarn. Bundle fiber strength is affected by fiber-to-fiber interactions in addition to the individual fiber strength. The bundle fiber strength is not always correlated with the strength measured from individual fibers and molecular mechanisms regulating fiber strength remain unknown. Gossypium hirsutum germplasm near isogenic lines (NILs), MD52ne obtained a gene promoting fiber strength became genetically almost identical to the MD90ne through back cross breeding. The molecular mechanisms behind the formation of superior individual fiber strength were unveiled by using these two NILs. Comparative fiber property analyses revealed that MD52ne have higher (~20%) fiber strength both single and bundle than its NIL MD90ne. Genome mapping analyses using F2 population of these two NILs discovered loci associated with high fiber strength is located on Upland cotton chromosome A03 at 84 mega base pairs. Comparative transcriptome analyses revealed that differential expressions of the genes involved in crystalline cellulose assembly, ethylene phytohormone and receptor-like kinase (RLK) signaling pathways between the MD52ne and MD90ne developing fibers. The superior BFS of MD52ne fibers resulted from high individual fiber strength with minor contributions from greater fiber length and the RLKs is candidate genes for regulating cotton fiber cell wall assembly and strength.