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ARS Home » Plains Area » Brookings, South Dakota » Integrated Cropping Systems Research » Research » Publications at this Location » Publication #330527

Research Project: Productive Cropping Systems Based on Ecological Principles of Pest Management

Location: Integrated Cropping Systems Research

Title: Genome-wide association mapping of soybean aphid resistance traits

Author
item Hanson, Anthony - University Of Minnesota
item Lorenz, Aaron - University Of Minnesota
item Bansal, Raman - The Ohio State University
item Bhusal, Siddhi - South Dakota State University
item Michel, Andy - The Ohio State University
item Hesler, Louis
item Koch, Robert - University Of Minnesota
item Jiang, Gou-liang - Virginia State University

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 3/19/2016
Publication Date: 3/20/2016
Citation: Hanson, A., Lorenz, A., Bansal, R., Bhusal, S., Michel, A., Hesler, L.S., Koch, R., Jiang, G. 2016. Genome-wide association mapping of soybean aphid resistance traits [abstract]. North Central Branch meeting, Entomological Society of America, June 5-9, 2016.

Interpretive Summary:

Technical Abstract: Soybean aphid is the most damaging insect pest of soybean in the Upper Midwest and is primarily controlled by insecticides. Soybean aphid resistance (i.e., Rag genes) has been documented in some soybean lines at chromosomes 6, 7, 13, and 16, but more sources of resistance are needed. Genome-wide association mapping (GWAS) can aid in identifying resistant lines to incorporate into breeding programs; phenotypic data is correlated with single nucleotide polymorphisms (SNPs) across a genome to identify SNPs associated with resistance and proximity to known resistance genes. Aphid counts from 1,599 soybean lines were collected from published studies, which used biotype 1 aphids and an aphid colony collected from South Dakota to screen for resistance. SNP data was obtained from previously genotyped lines in the USDA Soybean Germplasm Collection. Nucleotides measured at 42,449 different SNPs and average aphid count for each soybean line were tested for correlation across soybean lines. We found 25 SNPs for biotype 1 aphids and 22 SNPs for the South Dakota aphids to be significantly correlated with aphid counts (adjusted p < 0.05). Significant SNPs were found on chromosomes: 1, 5-14, 16, and 18-19. We identified a range of previously undescribed locations within different chromosomes that correlate with soybean aphid resistance. These SNPs can be used to determine lines likely to have aphid resistance traits before conducting formal screening studies. Focusing future experiments on novel chromosomal regions we identified as being associated with soybean aphid resistance could also help expedite identification of new Rag genes.