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Research Project: Integrated Research to Improve On-Farm Animal Health in Salmonid Aquaculture

Location: Cool and Cold Water Aquaculture Research

Title: Draft genome sequence of Lactococcus garvieae str. PAQ102015-99, an outbreak strain isolated from a commercial trout farm in the Northwestern United States

Author
item Nelson, Mike - University Of Connecticut
item Varney, Jed - Pacific Aquaculture
item Welch, Timothy - Tim
item Graf, Joerg - University Of Connecticut

Submitted to: Genome Announcements
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 6/10/2016
Publication Date: 8/4/2016
Publication URL: http://handle.nal.usda.gov/10113/63172
Citation: Nelson, M., Varney, J., Welch, T.J., Graf, J. 2016. Draft genome sequence of Lactococcus garvieae str. PAQ102015-99, an outbreak strain isolated from a commercial trout farm in the Northwestern United States. Genome Announcements. 4(4):e00781-16. https://doi.org/10.1128/genomeA.00781-16.
DOI: https://doi.org/10.1128/genomeA.00781-16

Interpretive Summary: Lactococcus garvieae is a commercially important pathogen in aquaculture as the causative agent of lactococcosis, which can cause up to 50% mortality in infected stocks and severe degradation of product quality. A commercial trout aquaculture facility reported an outbreak of lactococcosis. We were able to isolate L. garvieae str. PAQ102015-99 from the infected rainbow trout (Oncorhynchus mykiss) and sequenced its draft genome sequence. The analysis of the genome aided in the selection of this strain for vaccine development by providing critical information about its virulence factors and surface characteristics.

Technical Abstract: We announce the draft genome assembly of Lactococcus garvieae str. PAQ102015-99, a recently isolated strain from an outbreak of lactococcosis at a commercial trout farm in the Northwestern US. The draft genome comprises 14 contigs totaling 2,068,357 bp with an N50 of 496,618 bp and average G+C content of 38%.