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ARS Home » Southeast Area » Griffin, Georgia » Plant Genetic Resources Conservation Unit » Research » Publications at this Location » Publication #329275

Research Project: Conservation, Characterization, and Evaluation of Plant Genetic Resources and Associated Information

Location: Plant Genetic Resources Conservation Unit

Title: Screening of abscisic acid insensative (ABI) and low phosphorous efficiency (LPE) mutants from some sequenced lines in the sorghum TILLING population

Author
item Wang, Ming
item Xin, Zhanguo
item Moon, Sarah
item Tischenko, Viktor - University Of Georgia
item Tonnis, Brandon
item Pinnow, David
item Yu, Jianming - Iowa State University
item Burke, John
item Pederson, Gary

Submitted to: Plant Biology
Publication Type: Abstract Only
Publication Acceptance Date: 5/9/2016
Publication Date: 7/10/2016
Citation: Wang, M.L., Xin, Z., Moon, S.S., Tischenko, V., Tonnis, B.D., Pinnow, D.L., Yu, J., Burke, J.J., Pederson, G.A. 2016. Screening of abscisic acid insensative (ABI) and low phosphorous efficiency (LPE) mutants from some sequenced lines in the sorghum TILLING population. Plant Biology. Paper No. 700-015-Z.

Interpretive Summary: Sorghum population for Targeting Induced Local Lesion IN Genome (TILLING) was generated from BTx623 in 2005 and publicly available in 2009. After releasing to the public, this population was intensively screened by morphological observation in the field and a number of mutants with useful traits were identified. However, this TILLING population has not been screened on a large scale with controlled chemical concentration in the lab. Recently, 256 lines have been sequenced and annotated by alignment with the reference genome of BTx623 and >1.8 million SNPs were identified. We took the advantage of these sequenced mutant lines and screened them under controlled chemical concentration (10 µM abscisic acid and 1.5 µM phosphorus, respectively). Several abscisic acid insensitive (ABI) mutants have been identified in vitro culture. The mutant seedlings from the in vitro tubes have been transferred to soil for morphological observation and setting new seeds for reconfirmation of the response to abscisic acid (ABA). The method for identification of low phosphorus efficiency (LPE) mutants has been established using liquid cultures. Now this hydroponic method is being compared with the solid-phase sand-aluminum culture system in the Envirotron (University of Georgia). The comparison results indicate that the sand-aluminum medium is more appropriate in simulating plant responses to low-P condition in soils than liquid cultures.

Technical Abstract: Sorghum population for Targeting Induced Local Lesion IN Genome (TILLING) was generated from BTx623 in 2005 and publicly available in 2009. After releasing to the public, this population was intensively screened by morphological observation in the field and a number of mutants with useful traits were identified. However, this TILLING population has not been screened on a large scale with controlled chemical concentration in the lab. Recently, 256 lines have been sequenced and annotated by alignment with the reference genome of BTx623 and >1.8 million SNPs were identified. We took the advantage of these sequenced mutant lines and screened them under controlled chemical concentration (10 µM abscisic acid and 1.5 µM phosphorus, respectively). Several abscisic acid insensitive (ABI) mutants have been identified in vitro culture. The mutant seedlings from the in vitro tubes have been transferred to soil for morphological observation and setting new seeds for reconfirmation of the response to abscisic acid (ABA). The method for identification of low phosphorus efficiency (LPE) mutants has been established using liquid cultures. Now this hydroponic method is being compared with the solid-phase sand-aluminum culture system in the Envirotron (University of Georgia). The comparison results indicate that the sand-aluminum medium is more appropriate in simulating plant responses to low-P condition in soils than liquid cultures.