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ARS Home » Midwest Area » Ames, Iowa » Corn Insects and Crop Genetics Research » Research » Publications at this Location » Publication #327799

Title: Dynamic transcriptome profiling of Bean Common Mosaic Virus (BCMV) infection in Common Bean (Phaseolus vulgaris L.)

item MARTIN, KATHLEEN - Former ARS Employee
item JUGPREET, SINGH - Oak Ridge Institute For Science And Education (ORISE)
item HILL, JOHN - Iowa State University
item WHITHAM, STEVEN - Iowa State University
item Cannon, Steven

Submitted to: BMC Genomics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 6/6/2016
Publication Date: 8/11/2016
Citation: Martin, K., Jugpreet, S., Hill, J.H., Whitham, S., Cannon, S.B. 2016. Dynamic transcriptome profiling of Bean Common Mosaic Virus (BCMV) infection in Common Bean (Phaseolus vulgaris L.). Biomed Central (BMC) Genomics. 17:613. doi:10.1186/s12864-016-2976-8.

Interpretive Summary: Viruses significantly reduce yields of bean production worldwide, including dry beans and fresh snap beans. One of the most wide-spread viruses affecting bean is "Bean common mosaic virus" (BCMV). This virus can cause yield losses of 50-100% in a bean crop. This study describes how genes in a susceptible bean variety respond to two different strains of BCMV. One of the variants produces moderate symptoms, while another virus strain (newly described in this study) produces severe symptoms in this bean variety. The study also reports a novel way that some genes in common bean respond to the virus. The genes are processed differently by the plant when under viral attack, becoming "alternatively spliced" to generate a form of the gene that is capable of responding to the viral attack. These results have implications for bean breeders and growers by identifying a group of symptoms in some bean varieties that indicate infection by a newly identified viral strain, and guiding decisions for planting and breeding in this important crop.

Technical Abstract: Bean common mosaic virus (BCMV) is widespread, with Phaseolus species as the primary host plants. Numerous BCMV strains have been identified on the basis of a panel of bean varieties that distinguish the pathogenicity types with respect to the viral strains. Here, we report the transcriptional responses of a widely susceptible variety of common bean (Phaseolus vulgaris L., cultivar ‘Stringless green refugee’) to two BCMV strains, in a time-course experiment. We also report the genome sequence of a previously unreported BCMV strain. The interaction with the known strain NL1-Iowa causes moderate symptoms, and the newly identified strain (Strain 2 or S2) causes severe symptoms. The transcriptional profiles of host plants infected with the two isolates are distinct, and involve numerous differences in splice forms in particular genes, and pathway specific expression patterns. The number of alternate splicing events increases between days 4 and 8 after inoculation in the moderate interaction, but some specific alternate splice events showed decrease after inoculation in the severe interaction.