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ARS Home » Southeast Area » Mayaguez, Puerto Rico » Tropical Crops and Germplasm Research » Research » Publications at this Location » Publication #327428

Research Project: Development and implementation of robust molecular markers and genetic improvement of common and tepary beans in Central America and Haiti

Location: Tropical Crops and Germplasm Research

Title: Evaluation of the tepary bean (Phaseolus acutifolius) diversity panel for response to the NL 3 strain of Bean Common Mosaic Necrosis Virus (BCMNV) and for biological nitrogen fixation with Bradyrhizobium strains

Author
item Vargas, Ana - University Of Puerto Rico
item Hart, John
item Estevez De Jensen, C - University Of Puerto Rico
item Porch, Timothy - Tim
item Beaver, J - University Of Puerto Rico

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 1/11/2016
Publication Date: N/A
Citation: N/A

Interpretive Summary: Aphid-transmitted Bean Common Mosaic Necrosis Virus (BCMNV) and Bean Common Mosaic Virus (BCMV) are potyviruses that are seed transmitted in tepary bean. Developing resistance to these viruses will be critical for expanding production in areas where they are endemic. Biological nitrogen fixation (BNF) also represents an important trait in tepary bean due to its effective nodulation with Bradyrhizobium strains. This study evaluated the response of 315 genotypes, from the CIAT and USDA collections, that compose the tepary diversity panel (TDP), for virus resistance and BNF related traits. Host reaction to NL3 strains was evaluated according to phenotypic symptoms known in common bean and by detecting the infection using the ELISA BCMV/BCMNV test. Tepary genotypes showed venal necrosis, local necrotic lesions, while 5 accessions had no reaction and no infection detected by ELISA were identified in the TDP. For BNF, superior nodulation according to measured parameters of nodules was identified with the Bradyrhizobium elkanii strain USDA 3254 in the group of cultivated accessions. The genetics of tepary response to NL3 BCMNV will be pursued and genotypes with a resistant response are being integrated as parents in the tepary breeding program as well as those with desirable nodulation characteristics.

Technical Abstract: Aphid-transmitted Bean Common Mosaic Necrosis Virus (BCMNV) and Bean Common Mosaic Virus (BCMV) are potyviruses that are seed transmitted in tepary bean. Developing resistance to these viruses will be critical for expanding production in areas where they are endemic. Biological nitrogen fixation (BNF) also represents an important trait in tepary bean due to its effective nodulation with Bradyrhizobium strains. This study evaluated the response of 315 genotypes, from the CIAT and USDA collections, that compose the tepary diversity panel (TDP), for virus resistance and BNF related traits. Host reaction to NL3 strains was evaluated according to phenotypic symptoms known in common bean and by detecting the infection using the ELISA BCMV/BCMNV test. Tepary genotypes showed venal necrosis, local necrotic lesions, while 5 accessions had no reaction and no infection detected by ELISA were identified in the TDP. For BNF, superior nodulation according to measured parameters of nodules was identified with the Bradyrhizobium elkanii strain USDA 3254 in the group of cultivated accessions. The genetics of tepary response to NL3 BCMNV will be pursued and genotypes with a resistant response are being integrated as parents in the tepary breeding program as well as those with desirable nodulation characteristics.