Skip to main content
ARS Home » Northeast Area » Washington, D.C. » National Arboretum » Floral and Nursery Plants Research » Research » Publications at this Location » Publication #326703

Research Project: NEW AND EMERGING VIRAL AND BACTERIAL DISEASES OF ORNAMENTAL PLANTS: DETECTION, IDENTIFICATION, AND CHARACTERIZATION

Location: Floral and Nursery Plants Research

Title: Complete genome sequence of a novel potyvirus, Callistephus mottle virus identified in Callistephus chinensis

Author
item Seo, Eun-young - Chungnam National University
item Lim, Seungmo - Korea Research Institute Of Bioscience And Biotechnology
item Hammond, John
item Moon, Jae Sun - Korea Research Institute Of Bioscience And Biotechnology
item Lim, Hyoun-sub - Chungnam National University

Submitted to: Archives of Virology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 7/26/2016
Publication Date: 8/1/2016
Citation: Seo, E., Lim, S., Hammond, J., Moon, J., Lim, H. 2016. Complete genome sequence of a novel potyvirus, Callistephus mottle virus identified in Callistephus chinensis. Archives of Virology. 161:3281-3283.

Interpretive Summary: Virus infections frequently cause reductions of both yield and quality, and may result in ornamental plants or cut flowers being rejected as unmarketable. China aster (Callistephus chinensis; family Asteraceae) is a popular annual ornamental plant used for cut flower production and as a bedding plant. Plants of China aster grown in Korea and showing a severe mottling symptom in the leaves were shown to be infected with a previously uncharacterized virus belonging to the genus Potyvirus, and for which the name Callistephus mottle virus is proposed. Identification of this newly-described potyvirus in China aster will allow producers and extension agents or diagnostic laboratories to screen for this new virus, and encourage examination of related ornamental plants of the family Asteraceae to minimize the potential impacts of this virus on crop yield and quality.

Technical Abstract: The complete genomic sequence of a novel putative member of the genus Potyvirus was detected from Callistephus chinensis (china aster) in South Korea. The genomic RNA consists of 9,859 nucleotides excluding the 3’ poly(A) tail. The Callistephus virus genome, which contains the typical open reading frame of potyviruses, encodes a putative large polyprotein of 3,154 amino acids. The Callistephus virus showed 67 % nucleotide sequence identity (57 % query coverage) and 54 % polyprotein amino acid sequence identity (89 % query coverage) to the most closely related potyvirus, Plum pox virus. Phylogenetic analyses of polyprotein and mature proteins indicated that the Callistephus virus is most closely related to Plum pox virus, Apium virus Y, Celery mosaic virus and Panax virus Y. These results suggest that the Callistephus virus is a novel member of the genus Potyvirus, tentatively named Callistephus mottle virus (CalMV).