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ARS Home » Plains Area » Clay Center, Nebraska » U.S. Meat Animal Research Center » Genetics, Breeding, and Animal Health Research » Research » Publications at this Location » Publication #322718

Research Project: Genetic and Biological Determinants Of Respiratory Diseases Of Ruminants

Location: Genetics, Breeding, and Animal Health Research

Title: Large genomic differences between Moraxella bovoculi isolates acquired from the eyes of cattle with conjunctivitis versus the deep nasopharynx of asymptomatic cattle

Author
item Dickey, Aaron
item Loy, John - University Of Nebraska
item Bono, James - Jim
item Smith, Timothy - Tim
item Apley, Mike - Kansas State University
item Lubbers, Brian - Kansas State University
item Dedonder, Keith - Kansas State University
item Capik, Sarah - Kansas State University
item Larson, Robert - Kansas State University
item White, Brad - Kansas State University
item Blom, Jochen - University Of Giessen
item Chitko Mckown, Carol
item Clawson, Michael - Mike

Submitted to: Veterinary Research
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 1/22/2016
Publication Date: 2/13/2016
Publication URL: http://handle.nal.usda.gov/10113/61887
Citation: Dickey, A.M., Loy, J.D., Bono, J.L., Smith, T.P.L., Apley, M.D., Lubbers, B.V., DeDonder, K.D., Capik, S.F., Larson, R.L., White, B.J., Blom, J., Chitko-McKown, C.G., Clawson, M.L. 2016. Large genomic differences between Moraxella bovoculi isolates acquired from the eyes of cattle with conjunctivitis versus the deep nasopharynx of asymptomatic cattle. Veterinary Research. 47:31.

Interpretive Summary: Moraxella bovoculi is a recently described bacterium that is associated with "pinkeye" in cattle. In this study, finished genomes were sequenced for seven M. bovoculi isolates: three that originated from the eyes of clinical pinkeye cases and four from the nasopharynx of asymptomatic cattle. Isolates that originated from clinical cases were profoundly different from those sampled from asymptomatic cattle in genome structure, gene content and polymorphism diversity and the two types placed into distinct genetic groups. These results suggest that there are genetically distinct strains of M. bovoculi that may not associate with pinkeye in cattle.

Technical Abstract: Moraxella bovoculi is a recently described bacterium that is associated with infectious bovine keratoconjunctivitis (IBK) or "pinkeye" in cattle. In this study, closed circularized genomes were generated for seven M. bovoculi isolates: three that originated from the eyes of clinical IBK bovine cases and four from the deep nasopharynx of asymptomatic cattle. Isolates that originated from clinical cases were profoundly different from those sampled from asymptomatic cattle in genome structure, gene content and polymorphism diversity and the two types placed into distinct phylogenetic groups. These results suggest that there are genetically distinct strains of M. bovoculi that may not associate with IBK.