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Research Project: Management of Temperate-Adapted Fruit, Nut, and Specialty Crop Genetic Resources and Associated Information

Location: National Clonal Germplasm Repository

Title: Comparative RNA-seq for the investigation of tolerance to Verticillium wilt in black raspberry

Author
item Bushakra, Jill
item Bassil, Nahla
item Weiland, Jerry
item Finn, Chad
item Vining, Kelly - Oregon State University
item Filichkin, Sergie - Oregon State University
item Dossett, Michael - British Columbia Blueberry Council
item Bryant, Douglas - Danforth Plant Science Center
item Mockler, Todd - Danforth Plant Science Center

Submitted to: Acta Horticulture Proceedings
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 6/18/2015
Publication Date: 5/19/2016
Citation: Bushakra, J., Bassil, N.V., Weiland, G.E., Finn, C.E., Vining, K., Filichkin, S., Dossett, M., Bryant, D., Mockler, T. 2016. Comparative RNA-seq for the investigation of tolerance to Verticillium wilt in black raspberry. Acta Horticulture Proceedings. 1133:103-114. doi: 10.17660/ActaHortic.2016.1133.16.

Interpretive Summary: The fungal pathogen that causes the disease verticillium wilt is known to affect many crop species including black raspberry. Little work has been done to study how the fungus and black raspberry interact and why some plants are tolerant to the disease. We compared the genes that were expressed by a black raspberry cultivar infected with the fungus to the genes expressed by the same cultivar that was not subjected to the fungus. We found several genes whose expression levels are different between the two treatments. We will use public databases to compare the DNA sequence of these candidate genes to other plant species that have shown tolerance to this destructive disease.

Technical Abstract: Verticillium dahliae Kleb., a cause of verticillium wilt, is a wide-spread, soil-borne fungal pathogen with a wide host range that includes many fruit and vegetable crops. Verticillium dahliae has been isolated from Rubus species showing symptoms of the disease. Very little is known about the interactions of V. dahliae and woody plants in general and Rubus in particular. We compared the transcriptome of black raspberry (Rubus occidentalis L.) cultivar Jewel inoculated with V. dahliae with that of uninfected ‘Jewel’ plants grown under the same greenhouse conditions. From this analysis, 207 genes were differentially expressed (DE) between Verticillium-inoculated and non-inoculated (control) ‘Jewel’ root transcriptomes. Eight of these genes had apparent homology to known or candidate genes involved in disease resistance. Fourteen genes with homology to transcription factors were among the DE genes.