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ARS Home » Pacific West Area » Pullman, Washington » Plant Germplasm Introduction and Testing Research » Research » Publications at this Location » Publication #317385

Research Project: Management of Plant Genetic Resources and Associated Information

Location: Plant Germplasm Introduction and Testing Research

Title: Identification of QTL controlling high levels of partial resistance to Fusarium solani f. sp. pisi in pea

Author
item Coyne, Clarice - Clare
item Pilet-nayel, Marie-laure - Institut National De La Recherche Agronomique (INRA)
item Mcgee, Rebecca
item Porter, Lyndon
item Smykal, Petr - Palacky University
item Grunwald, Niklaus - Nik
item Inglis, Debra - Washington State University Extension Service

Submitted to: Plant Breeding
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/8/2015
Publication Date: 6/29/2015
Citation: Coyne, C.J., Pilet-Nayel, M., McGee, R.J., Porter, L., Smykal, P., Grunwald, N.J., Inglis, D. 2015. Identification of QTL controlling high levels of partial resistance to Fusarium solani f. sp. pisi in pea. Plant Breeding. 134:446-453.

Interpretive Summary: Fusarium root rot is a common biotic restraint on pea yields worldwide and genetic resistance is the most feasible method for improving pea production. This study was conducted to discover the gene regions (called QTL) controlling high levels of genetic resistance to Fusarium root rot caused by Fusarium solani f.sp. pisi. A recombinant inbred line population from the cross Dark Skin Perfection × 90-2131 was screened in a Fusarium root rot field disease nursery for three years. Composite interval mapping was employed for gene region detection using the means of disease severity from three environments. The QTL analysis identified five regions (QTL), including one QTL identified in all three years. The multi-year QTL called Fsp-Ps2.1 contributed to a significant portion of the resistance, from 22.1% up to 72.2%. A second QTL, Fsp-Ps6.1, contributed 17.3% of the resistance. The other three QTL are of additional interest as they co-locate with previously reported pea-Fusarium root rot resistance QTL regions. Four QTL are flanked by breeder-friendly co-dominate SSRs and may be useful in marker-assisted breeding of pea for high levels of resistance to Fusarium root rot.

Technical Abstract: Fusarium root rot is a common biotic restraint on pea yields worldwide and genetic resistance is the most feasible method for improving pea production. This study was conducted to discover quantitative trait loci (QTL) controlling genetic partial resistance to Fusarium root rot caused by Fusarium solani (Mart.) Sacc. f.sp. pisi (F.R. Jones) W.C. Snyder & H.N. Hans (Fsp). A recombinant inbred line population from the cross Dark Skin Perfection × 90-2131 was screened in a Fusarium root rot field disease nursery for three years. Composite interval mapping was employed for QTL detection using the means of disease severity from three environments. The QTL analysis identified five QTL, including one QTL identified in all three environments. The multi-environment QTL Fsp-Ps2.1 contributed to a significant portion of the phenotypic variance (22.1-72.2%) while a second QTL, Fsp-Ps6.1, contributed 17.3% of the phenotypic variance. The detection level (LOD 2.9) for QTL Fsp-Ps6.1 was not reached in the other two environments (LOD 2.61, 2.62). The other single environment QTL are of additional interest as they co-locate with previously reported pea-Fusarium root rot resistance QTL regions. QTL Fsp-Ps2.1, Fsp-Ps3.1, Fsp-4.1 and Fsp-Ps7.1 are flanked by co-dominate SSRs and may be useful in marker-assisted breeding of pea for high levels of partial resistance to Fsp.