Location: Fruit and Tree Nut ResearchTitle: Sequencing analysis of wood-decay fungi associated with peach scaffold breakage
Submitted to: American Society of Horticulture Science Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 4/24/2015
Publication Date: 4/24/2015
Citation: Chen, C., Bock, C.H., Cottrell, T.E. 2015. Sequencing analysis of wood-decay fungi associated with peach scaffold breakage [abstract]. American Society of Horticulture Science. Paper No. 21463.
Interpretive Summary: Peach is an important fruit crop worldwide. Peach tree health and longevity are affected by many biotic and abiotic stresses. Some peach diseases are well characterized and managed, but it remains unclear what and how ‘white-rot’ lignicolous fungi colonize in peach and affect the long-term health of peach trees. The rot in scion scaffold heartwood may be an additional cause of scaffold limb break and short orchard longevity in the southeastern states (8-10 years compared to 17-20 years in the western states). To remain sustainable and profitable, tree health and orchard longevity must be maximally prolonged. This study is to compare the fungal growth between scion and rootstock cultivars, and to determine the identities of these fungi using sequencing technologies. The results will facilitate further studies on the epidemiology, colonization process, disease progress and management.
Technical Abstract: Breakage of healthy-looking scaffold limbs is commonly seen in peach orchards in central Georgia, in the United States, and may have impact on the health and longevity of peach trees. White fungal mycelia were observed on the broken surface of some newly snapped, but otherwise healthy-looking peach scaffold limbs. Cross-cut limb sections from apparently healthy scion cultivars were collected and incubated under high humidity for 2 weeks. Fungal growth was observed on the cross-cut surface in two repeated experiments. In this study, genomic DNAs were extracted from the fungus growing on surface of the cross-cut sections. Using next-generation sequencing technology and metagenomic analysis, two white-rot fungi, Trametes versicolor and Schizophyllum commune, were deemed to be the most likely fungi colonizing the heartwood in these specimens. The former likely was predominant over the latter partly because there was a much greater number of read alignments onto the sequences from the T. versicolor genome than that from the S. commune genome. The co-existence of the two fungi were confirmed by Sanger sequencing of an internal transcribed spacer (ITS) amplicon and amplicons from new primers designed from selected contigs, as two different fragments of similar sizes were amplified by the ITS and most selected primers from the DNA extracted from the specimens. Possible reasons for the white-rot fungi colonizing and growing within the peach limbs of apparently healthy, living trees are discussed, as are the likely impact of the colonization on the peach tree health. Further research needs are considered.