Location: Floral and Nursery Plants ResearchTitle: Detection of Helenium virus S and two distinct isolates of Butterbur mosaic virus in a single plant of Veronica Author
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 5/5/2015
Publication Date: N/A
Technical Abstract: A Veronica plant showing mosaic symptoms was examined by electron microscopy, which revealed particles typical of carlaviruses. RNA extracted from virions partially purified by high speed centrifugation through a 30% sucrose cushion was used as template for random PCR to produce a viral cDNA library; six distinct cloned amplicons were sequenced. Sequences revealed similarity to Butterbur mosaic virus (ButMV) coat protein (CP) and replicase (RdRp), and Helenium virus S (HelVS) RdRp, while another RdRp fragment showed distant relationships to different carlaviruses. When cDNA was produced using a tagged oligo-dT primer, and the tag primer paired for PCR with a degenerate potexvirus/carlavirus primer, products of a size consistent with carlaviruses were cloned; sequence analysis identified HelVS and ButMV. Use of a ButMV-specific TGB2 forward primer (based on the genome of the Japanese isolate, ButMV-J) yielded an additional ButMV clone distinct from the first. Veronica ButMV-A and ButMV-B have 78% nt identity to each other, and 82% and 78% nt identity to the full genome of ButMV-J (AB517596). Veronica HelVS has 98% identity to HelVS from the UK (D10474, S71594). The recovery of two distinct clones each of the RdRp and 3’-terminal regions of ButMV; RdRp and 3’-terminal regions of HelVS; and an RdRp clone of as yet unidentified carlavirus from Veronica indicate the presence of a complex mixed infection in this plant. ButMV has previously been reported only from Japan.