|BAMPI, DAIANA - Universidade Estadual Paulista (UNESP)|
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 5/8/2015
Publication Date: N/A
Technical Abstract: The genus Allium includes important species used for human consumption and many other species grown as ornamentals. Plants of the genus Allium can be infected by several viruses of the genera Allexivirus, Carlavirus and Potyvirus, often in mixed infections. Bulbs of fifteen different species of Allium were purchased from retail nurseries and planted in the field in the fall of 2013. Leaf tissue from the flowering symptomatic plants were collected in spring 2014, and tested by PCR using generic primers for the genus Allexivirus, Carlavirus and Potyvirus. PCR-positive samples were re-tested using specific primers for Leek yellow stripe virus (LYSV) Onion yellow dwarf virus (OYDV), Shallot latent virus (SLV), Garlic common latent virus (GarCLV), Garlic virus A (GarVA), Garlic virus B (GarVB), Garlic virus C (GarVC), Garlic virus D (GarVD), Garlic virus E (GarVE), Garlic mite-borne filamentous virus (GarMbFV) and Shallot virus X (ShVX). After flowering and foliage dieback, the bulbs were harvested and stored at 4º C for 60 days, before being planted in pots and maintained in a cooler until leaves emerged, and then transferred to a greenhouse. The sprouted plants were tested by ELISA using antibodies specific for the viral species described above. ELISA confirmed PCR detection of GarVB, GarVC, GarVD, GarVE and ShVX in Allium sphaerocephalon and Allium caeruleum, LYSV in Allium bulgaricum and Allium atropurpureum, and SLV in Allium moly.