Submitted to: International Symposium on Genetics in Aquaculture
Publication Type: Abstract Only
Publication Acceptance Date: 3/2/2015
Publication Date: 6/21/2015
Citation: Liu, S., Vallejo, R.L., Palti, Y., Gao, G., Marancik, D.P., Wiens, G.D. 2015. Identification of single nucleotide polymorphism markers associated with bacterial cold water disease resistance and spleen size in rainbow trout. International Symposium on Genetics in Aquaculture. 624.P403.
Technical Abstract: Bacterial cold water disease (BCWD) is one of the frequent causes of elevated mortality in salmonid aquaculture. Previously, we identified and validated microsatellite markers associated with QTL (quantitative trait loci) for BCWD resistance and spleen size in rainbow trout. The objective of this study was to identify single nucleotide polymorphism (SNP) markers associated with BCWD resistance and spleen size using both genome-wide association studies (GWAS) and QTL mapping approaches. A total of 298 offspring from the two half-sib families used in our previous study to validate the significant BCWD QTL on chromosome Omy19 were genotyped with RAD-seq (restriction-site-associated DNA sequencing), and 7,859 informative SNPs were identified. Based on GWAS, 18 SNPs associated with BCWD resistance and 20 SNPs associated with spleen size were identified. QTL mapping revealed three significant QTL for BCWD resistance. In addition to the previously validated dam-derived QTL on chromosome Omy19, two significant BCWD QTL derived from the sire were identified on chromosomes Omy8 and Omy25. Consistent with previous study, there was no spleen size QTL on chromosome Omy19 in these two families. However, one significant QTL for spleen size on chromosome Omy2 was identified. The SNP markers reported in this study would facilitate fine mapping to identify positional candidate genes for BCWD resistance in rainbow trout.