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ARS Home » Pacific West Area » Pullman, Washington » Plant Germplasm Introduction and Testing Research » Research » Publications at this Location » Publication #313222

Title: Len Gen: The international lentil genome sequencing project

item BETT, KIRSTIN - University Of Saskatchewan
item RAMSAY, LARISSA - University Of Saskatchewan
item CHAN, CRYSTAL - University Of Saskatchewan
item SHARPE, ANDREW - Saskatoon Research Center
item COOK, DOUGLAS - University Of California
item PENMETSA, VARMA - University Of California
item CHANG, PETER - University Of Southern California
item Coyne, Clarice - Clare
item McGee, Rebecca
item MAIN, DORRIE - Washington State University
item VANDENBERG, BERT - University Of Saskatchewan

Submitted to: Plant and Animal Genome Conference
Publication Type: Abstract Only
Publication Acceptance Date: 10/1/2014
Publication Date: 1/10/2015
Citation: Bett, K., Ramsay, L., Chan, C., Sharpe, A., Cook, D., Penmetsa, V., Chang, P., Coyne, C.J., McGee, R.J., Main, D., Vandenberg, B. 2015. Len Gen: The international lentil genome sequencing project. Plant and Animal Genome Conference.

Interpretive Summary:

Technical Abstract: We have been sequencing CDC Redberry using NGS of paired-end and mate-pair libraries over a wide range of sizes and technologies. The most recent draft (v0.7) of approximately 150x coverage produced scaffolds covering over half the genome (2.7 Gb of the expected 4.3 Gb). Long reads from PacBio sequencing are being used to fill gaps and perform superscaffolding. Genotype by sequencing (GBS) in key mapping populations is being used to anchor scaffolds as well as select individuals for re-sequencing. Collaborations with labs in Australia, Czech Republic and ICARDA will facilitate further assembly and annotation of the genome as well as add to the growing database of genotypic diversity in the global lentil germplasm. The sequence data from initial assemblies has been sufficient for the identification of gene sequences for traits of interest in the lentil breeding program and derived SNP markers are now being used in routine MAS.