Location: Corn Insects and Crop Genetics ResearchTitle: Characterization of twelve novel microsatellite markers of Sogatella furcifera (Horváth) (Hemiptera: Delphacidae) identified from next generation sequence data Author
|Nam, Hwa Yeun - Seoul National University|
|Kim, Kyung Seok - Seoul National University|
|Lee, Joon-ho - Seoul National University|
Submitted to: Journal of Insect Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 6/10/2015
Publication Date: 7/10/2015
Citation: Nam, H., Coates, B.S., Kim, K., Lee, J. 2015. Characterization of twelve novel microsatellite markers of Sogatella furcifera (Horváth) (Hemiptera: Delphacidae) identified from next generation sequence data. Journal of Insect Science. 15(1):94. DOI: 10.1093/jisesa/iev069.
Interpretive Summary: The white-backed planthopper is a highly destructive insect pest in China and Southeast Asia, and has long-range migration patterns analogous to those found in several corn pests in the United States. A developmental project between an ARS Scientist and collaborators demonstrated molecular genetic markers called microsatellites could be isolated from random genomic DNA fragments derived from next generation sequencing platforms. This method allowed for comparatively cost effective means to generate DNA sequence data for downstream marker development. The procedures and bioinformatic methods can be applied by government, university and private sector scientists for the rapid development of genetic markers.
Technical Abstract: The white-backed planthopper, Sogatella furcifera (Horváth) (Hemiptera: Delphacidae), is a major pest of rice and has long-range migratory behavior in Asia. Microsatellite markers (simple sequence repeats, SSRs) have been widely used to determine the origins and genetic diversity of insect pests. We identified novel microsatellite loci for S. furcifera samples collected from Laos, Vietnam and three localities in Bangladesh from next-generation sequencing platform data. Of these, size polymorphism at 12 microsatellite loci were verified for 40 adult individuals collected from Shinan, South Korea. The average number of alleles per locus was 7.92. The mean values of observed (HO) and expected heterozygosities (HE) were 0.615 and 0.757 respectively. These new microsatellite markers will be a resource for future ecological genetic studies of S. furcifera samples across more broad geographic regions in Asia and may assist in estimations of genetic differentiation and gene flow among populations for implementation of more effective management strategies to control this serious rice pest.