|Welch, Timothy - Tim|
|Leeds, Timothy - Tim|
|Wiens, Gregory - Greg|
Submitted to: Plant and Animal Genome Conference
Publication Type: Abstract Only
Publication Acceptance Date: 11/18/2013
Publication Date: 1/12/2014
Citation: Vallejo, R.L., Palti, Y., Liu, S., Marancik, D.P., Welch, T.J., Leeds, T.D., Evenhuis, J., Rexroad III, C.E., Wiens, G.D. 2014. Validation of linkage between BCWD resistance and spleen size QTL on Omy19 in rainbow trout: Pleiotropy versus linkage. Plant and Animal Genome Conference. 361.
Technical Abstract: Bacterial cold water disease (BCWD) is caused by infection with Flavobacterium psychrophilum, and results in significant economic losses in salmonid aquaculture. Previously, we identified a major QTL for BCWD resistance on Omy19 (h2q=0.57-0.67) as well as a QTL for surrogate measures of disease resistance (SW=spleen weight and SI=spleen index). The objectives of this study were to (1) validate the observed linkage of BCWD resistance with spleen size in an additional genetic background; and (2) test the hypothesis of “close linkage” versus “complete pleiotropy”. We developed four full-sib families whose offspring were evaluated for BCWD resistance, SW and body weight. A total of 800 offspring were genotyped with eight STR markers from Omy19. We estimated genetic correlation between BCWD resistance and spleen size by fitting a multivariate mixed model. We then ran univariate and bivariate linkage analysis using half-sib family regression analysis with GridQTL and variance components method with Solar. We estimated positive non-significant genetic correlation rg(survdays, SW)=0.21 with heritability h2survdays=0.42 and h2SW=0.65. We validated significant and suggestive QTL for BCWD resistance (11-13 cM; h2q=0.03-0.21) and spleen size (8-33 cM; h2q=0.06-0.31) on Omy19. The bivariate linkage analysis of survstatus with SW detected a suggestive QTL (LOD=1.77; h2q=0.07) at 34 cM. None of the “close linkage” and “complete pleiotropy” models were rejected at P=0.05 significance level. Although non-significant, the log likelihood of “complete pleiotropy” models were higher than those of “close linkage” models at 0-25 cM region which provides a weak support for a pleiotropic QTL.