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ARS Home » Midwest Area » Ames, Iowa » National Animal Disease Center » Virus and Prion Research » Research » Publications at this Location » Publication #300100

Title: Hemagglutinin inhibition assay with swine sera

item Kitikoon, Pravina
item GAUGER, PHILLIP - Iowa State University
item Baker, Amy

Submitted to: Methods in Molecular Biology
Publication Type: Book / Chapter
Publication Acceptance Date: 3/10/2014
Publication Date: 6/30/2014
Citation: Kitikoon, P., Gauger, P.C., Vincent, A.L. 2014. Hemagglutinin inhibition assay with swine sera. In: Spackman, E., editor. Methods in Molecular Biology, Animal Influenza Virus. 2nd edition. New York, NY: Springer. p. 295-301.

Interpretive Summary:

Technical Abstract: Hemagglutination is based on the ability of certain viruses to agglutinate red blood cells (RBC) of certain animal species by formation of cross-linking lattices between RBC. Antibodies that have the ability to inhibit the hemagglutination property of influenza A viruses are generally thought to protect from infection. The hemagglutination-inhibition (HI) test is a serological assay that measures the titer of specific antibodies in the sera and is the most common serological assay used to detect anti-influenza antibodies in swine sera.