Submitted to: American Society for Microbiology
Publication Type: Other
Publication Acceptance Date: 5/2/2013
Publication Date: 5/18/2013
Citation: Harhay, G.P. 2013. The methylome and virulence of bovine respiratory disease bacterial pathogens. American Society for Microbiology. Presentation in WS-24, Studying Whole-Genome Microbial Epigenetics. Interpretive Summary:
Technical Abstract: With the advent of single molecule, real-time (SMRT®) sequencing, it is now possible to study complete microbial epigenomes. It has been known for decades that methylation and other types of epigenetic modifications in bacteria are responsible for much more than restriction-modification mechanics, but genome-scale study of most modifications, such as methylated adenine, has been impossible. Methylation is also associated with gene expression and phase variation, replication, virulence, and more. Speakers will describe how to apply whole-epigenome studies to the understanding of microbial biochemistry and function. Systematic approaches combining complete de novo assemblies and annotations, methylation, and transcriptomes will be discussed. The basic principles of SMRT® Sequencing as they apply to epigenetics will also be covered.