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Title: Impact of parenteral lipid emulsions on metabolomic phenotype in preterm TPN-fed piglets

Author
item KULKARNI, MADHULIKA - Children'S Nutrition Research Center (CNRC)
item VLAARDINGERBROEK, HESTER - Sophia Children'S Hospital
item STOLL, BARBARA - Children'S Nutrition Research Center (CNRC)
item ILKAYEVA, OLGA - Duke University Medical Center
item NEWGARD, CHRISTOPHER - Duke University Medical Center
item OLUTOYE, OLUYINKA - Children'S Nutrition Research Center (CNRC)
item VAN GOUDOEVER, JOHANNES - University Of Amsterdam
item Burrin, Douglas - Doug

Submitted to: Pediatric Research
Publication Type: Abstract Only
Publication Acceptance Date: 4/11/2013
Publication Date: 5/4/2013
Citation: Kulkarni, M.A., Vlaardingerbroek, H., Stoll, B., Ilkayeva, O., Newgard, C., Olutoye, O., Van Goudoever, J.B., Burrin, D.G. 2013. Impact of parenteral lipid emulsions on metabolomic phenotype in preterm TPN-fed piglets [abstract]. Pediatric Research. E-PAS2013:3830.483.

Interpretive Summary:

Technical Abstract: Lipids in parenteral nutrition provide essential fatty acids and are a major source of energy for hospitalized neonates. Intralipid (IL) is the only approved lipid emulsion in the US, but new generation emulsions include Omegaven (OV) and SMOFlipid (SL). There are no studies describing the metabolite profile in neonates comparing different type of lipid emulsions. Our objective was to perform metabolomic profiling of liver and muscle tissue in total parenteral nutrition (TPN) fed piglets given different lipid emulsions. Preterm pigs were assigned to 4 groups (7-14 pigs per group; equal daily macronutrient intake with 5 g per kg lipid): PN+IL, PN+OV, PN+SL, and an enteral group fed milk formula (EN). Plasma, muscle, and liver tissue were collected after 14 d and subjected to analysis of fatty acid, amino acid, and citric acid cycle metabolites by various HPLC, GC, and MS methods. Results showed that in liver tissue, acyl-CoA species were most abundant for the dominant fatty acids in the respective lipid emulsions. Tissue-free carnitine in EN pigs was 4-fold higher than TPN groups, and this resulted in a reduced liver, but not muscle acyl-carnitine levels. The acyl-CoA: acyl-carnitine ratios were higher in liver than muscle tissue and also higher in all TPN groups vs EN. Among citric acid cycle intermediates, only citrate was higher in EN vs all TPN groups. We conclude that metabolomic profiles revealed a carnitine deficiency in TPN groups that suggests impaired hepatic fatty acid oxidation.