|QUESENBERRY, KENNETH - University Of Florida|
Submitted to: Book Chapter
Publication Type: Book / Chapter
Publication Acceptance Date: 10/5/2013
Publication Date: 9/13/2014
Citation: Sullivan, M.L., Quesenberry, K.H. 2014. Clover, Red (Trifolium pretense). In: Wang, K., editor. Agrobacterium Protocols. 3rd edition. New York, NY:Springer Science+Business Media. p. 237-254.
Technical Abstract: Genetic modification of plants by the insertion of transgenes can be a powerful experimental approach to answer basic questions about gene product function. This technology can also be used to make improved crop varieties for use in the field. To apply this powerful tool to red clover, an important forage legume, a population of red clover with high potential for regeneration in tissue culture has been developed. Here we provide a detailed procedure for Agrobacterium-mediated transformation of genotypes derived from this regenerable population. We have successfully used this methodology to express a beta-glucuronidase (GUS) reporter gene and for hairpin RNA-mediated silencing of endogenous genes for polyphenol oxidase and a transferase crucial in phaselic acid accumulation.