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United States Department of Agriculture

Agricultural Research Service


Location: Dietary Prevention of Obesity-related Disease Research

Title: A high protein diet upregulated whole-body protein turnover during energy deficit

item Margolis, Lee
item Cao, Jay
item Sauter, Edward
item Whigham Grendell, Leah
item Mcclung, James
item Combs, Gerald
item Young, Andrew
item Pasiakos, Stefan

Submitted to: Federation of American Societies for Experimental Biology Conference
Publication Type: Abstract Only
Publication Acceptance Date: 11/14/2011
Publication Date: 9/29/2012
Citation: Margolis, L.M., Cao, J.J., Sauter, E.R., Whigham Grendell, L.D., Mcclung, J.P., Combs, G.F., Young, A.J., Pasiakos, S.M. 2012. A high protein diet upregulated whole-body protein turnover during energy deficit. Federation of American Societies for Experimental Biology Conference. 26:1013.3.

Interpretive Summary:

Technical Abstract: The effects of higher protein diets and sustained energy deficit (ED) on whole-body protein turnover (WBPTO) are not well described. This study examined whether dietary protein level influences whole-body protein breakdown (Ra), non-oxidative leucine disposal (NOLD), and oxidation (Ox) during ED. Using a randomized-block design, 24 males and five females (21 ± 3 yr) participated in a controlled trial. Volunteers consumed either standard (SP; 0.8 g•kg-1•d-1), moderate (MP; 1.6 g•kg-1•d-1), or high (HP; 2.4 g•kg-1•d-1) protein diets for 31 days. A weight maintenance (WM, days 1-10) period was followed by 21 days of ED (days 11-31) equal to 40% of total daily energy expenditure. Resting WBPTO (µmol•kg-1·h-1) was determined during WM (day 10) and ED (day 31) using primed, continuous [13C]-leucine infusions. Volunteers lost (P < 0.05) ~ 3.3 ± 1 kg in response to ED regardless of protein level. Ra was decreased (P < 0.05) in response to ED for SP and MP diets, but did not change for HP. NOLD was decreased (P < 0.05) following ED regardless of protein level. Ox increased linearly between SP (9.7 ± 0.5), MP (11.8 ± 0.5), and HP (13.9 ± 0.5) diets during WM, with increased Ox observed only for HP (16 ± 0.7) during ED (diet-by-energy interaction, P < 0.05). These data demonstrate that sustained ED and level of dietary protein intake modulate WBPTO, as consumption of a high protein diet upregulates WBPTO during ED.

Last Modified: 10/16/2017
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