|YANG, LUMING - University Of Wisconsin|
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 10/24/2011
Publication Date: 10/28/2011
Citation: Yang, L., Weng, Y. 2011. Map-based cloning a multifunctional gene regulating leaf, flower and fruit development and plant architecture in cucumber [abstract]. 2011 Plant Sciences Symposium.
Technical Abstract: standard-leaf type cucumbers as well as multi-branched vines not presented in standard-leaf type cucumbers. Littleleaf has the potential to address some problems in picking cucumber production. Littleleaf is controlled by a single, recessively inherited gene designated ll. In the present study, a high resolution map was developed using 145 recombinant inbred lines (RILs) and 946 F2 plants developed from cucumber inbred lines H19 (little leaf) and the gynoecious, standard leaf inbred line G421. In total, 138 SSR markers were developed in the region of Chromosome 6 previously defined by two SSR marerks. Six more closely linked markers flanking the littleleaf locus were mapped. Based on 946 F2 plants, SSR marker 11B5 was 0.92 cM and 13A11 was 0.67 cM away from the ll gene, respectively. Physically, this genetic distance covered approximate 560 kb. Among the 50 open read frames (ORFs) predicted in this region, four were considered as potential candidates for the ll locus. Equencing of the 4 ORFs indicated that only one had genomic DNA level polymorphism (SNP), which was functionally to the sap gene in Arabidopsis.