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United States Department of Agriculture

Agricultural Research Service

Research Project: Innovative Bioresource Management Technologies for Enhanced Environmental Quality and Value Optimization

Location: Coastal Plain Soil, Water and Plant Conservation Research

Title: Submission to GenBank of full 16S rRNA gene sequence of novel anammox bacterium Candidatus "Brocadia caroliniensis" NRRL B-50286

item Vanotti, Matias
item Szogi, Ariel
item Rothrock, Michael

Submitted to: National Center for Biotechnology Information (NCBI)
Publication Type: Popular Publication
Publication Acceptance Date: 3/3/2011
Publication Date: 4/12/2011
Citation: Vanotti, M.B., Szogi, A.A., Rothrock Jr, M.J. 2011. Submission to GenBank of full 16S rRNA gene sequence of novel anammox bacterium Candidatus "Brocadia caroliniensis" NRRL B-50286. National Center for Biotechnology Information (NCBI). Available:

Interpretive Summary:

Technical Abstract: Disclosed is a novel anammox bacteria isolate Candidatus Brocadia caroliniensis, having Accession Deposit Number NRRL B-50286, and the characteristics of oxidizing ammonia and releasing di-nitrogen under anaerobic conditions. Also disclosed are methods for treating wastewater using said anammox bacteria isolate. The novel anammox bacteria was isolated from livestock manure sludges at the ARS laboratory in Florence, South Carolina. This bacteria is capable of reactivation after lyophilization. The high nitrogen removal rate obtained with NRRL B-50286 of more than 1.7 kg N/m3 reactor/day is optimum for wastewater treatment applications. The novel bacterial strain Candidatus Brocadia caroliniensis of this invention (Serial No. 13/013,874 – Filed January 26, 2011) may be used for the treatment of wastewater having undesirable levels of ammonia, including agricultural, industrial, or municipal wastewaters. Compared to conventional biological nitrogen removal methods, the anammox process can save more than 50% of the oxygen supply and 100% of the external carbon source for denitrification. This leads to a significant decrease in operational costs. In addition, by-products of anammox do not include greenhouse gases. ARS seeks a commercial partner to bring this technology to market.

Last Modified: 10/15/2017
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