Location: Children's Nutrition Research CenterTitle: Polymorphisms in the BCE and ECR3 regions on BTA6 and association analysis with production and management traits in dairy cattle Author
Submitted to: Annual International Plant & Animal Genome Conference
Publication Type: Abstract Only
Publication Acceptance Date: 12/22/2008
Publication Date: 12/22/2008
Citation: Rincon, G., Rijnkels, M., Medrano, J.F. 2008. Polymorphisms in the BCE and ECR3 regions on BTA6 and association analysis with production and management traits in dairy cattle. In: Annual International Plant & Animal Genome Conference, January 10-14, 2009, San Diego, California. P475. Interpretive Summary:
Technical Abstract: Beta-casein enhancer (BCE) and evolutionarily conserved region 3 (ECR3) are potential regulatory regions located in the casein gene cluster on BTA6. In the present study we resequenced BCE and ECR3 genomic regions in a sample of 8 Brown Swiss, 8 Holstein and 8 Jersey cows that were unrelated in a three generation pedigree. Three SNPs were found in the BCE region (SNP_20586, SNP_20752, SNP_20827) and two SNPs were found in the ECR3 region (SNP_9109, SNP_9518). The SNPs were genotyped and tested for associations with milk production and management traits in 800 Holstein bulls from the UC Davis archival collection and 300 Holstein cows from farms in the California Central Valley. Bull PTA values were obtained from the USDA Animal Improvement Programs Laboratory (Beltsville, MD). Milk composition analysis was performed to obtain phenotypes in the cow population. The association analysis was developed using the Golden Helix Regression Module from Helixtree to test allelic associations with phenotypic variables. Significant results (p<0.01; FDR<0.05) were observed for SNP_20752 in the bull population for milk yield, protein yield, productive life and somatic cell score PTA traits. The same SNP was also significantly associated with protein and casein percentages in the cow population. SNP_20586 and SNP_9109 were associated with PTA protein percentage in bulls. These results emphasize the importance of SNP variation in the BCE and ECR3 regions in relation to milk production and management traits in dairy cattle.