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Title: Additional pea EST-SSR markers for comparative mapping in pea (Pisum sativum L.)

item DECARIE, J - Louisiana Technical University
item Coyne, Clarice - Clare
item BRUMETT, S - Louisiana Technical University
item SHULTZ, J - Louisiana Technical University

Submitted to: Plant Breeding
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 9/1/2011
Publication Date: 11/17/2011
Citation: Decarie, J., Coyne, C.J., Brumett, S., Shultz, J. 2011. Additional pea EST-SSR markers for comparative mapping in pea (Pisum sativum L.). Plant Breeding. DOI: 10,1111/j.1439-0523.2011.01917.x.

Interpretive Summary: Pea is a widely grown vegetable and the second most important grain legume grown in the world. Increasing the density of gene-based markers on the linkage map of pea serves an important role in the genomic-assisted breeding efforts of improving the quality and production of this healthful and delicious food legume. Markers based on genes allow pea breeders to leverage the genomic (sequence) knowledge of sequenced legume crops (e.g. soybean) to assist in the genetic improvement of under-resourced legume crops (e.g. pea). We report the development of 45 new gene-based markers (EST-SSRs) for comparative mapping of economic traits and the map location of seven gene-based markers for use in pea improvement programs.

Technical Abstract: The availability of a large set of molecular markers is an excellent resource for both applied and basic research for any organism. The current consensus molecular map of pea is 1,430 cM and contains 239 simple sequence repeat markers. The goal was to identify and test unique gene-based simple sequence repeat markers to increase the available number for comparable mapping with sequenced legume species. A total of 6,327 mRNA sequences were screened using a JAVA algorithm designed to report all 1-6 bp repeats, culminating in the design of 96 novel, gene-based simple sequence repeat markers. The markers were tested for polymorphism between the parents of two recombinant inbred line mapping populations (Early Freezer 680 x PI 269818 and C580257A x 74SN3A) and 11 additional pea lines. A total of 45 new EST-SSR polymorphic markers were identified using these test lines, another 35 that were monomorphic and 16 that failed or produced sub-standard amplification profiles within the tested genotypes. Linkage map locations are presented for seven of the new EST-SSR markers on the consensus pea map. Primer sequence, repeat type, status within the tested genotypes and the SSR-finding algorithm are presented. The forty-five polymorphic EST-SSR markers identified in this research will add to the density of gene-based linkage map Pisum sativum improving the power of comparative legume mapping.